Article

Das BK, Gentsch JR, Cicirello HG, et al. Characterization of rotavirus strains from newborns in New Delhi, India

Department of Paediatrics and Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi.
Journal of Clinical Microbiology (Impact Factor: 4.23). 08/1994; 32(7):1820-2.
Source: PubMed

ABSTRACT Between 1986 and 1993, 72% of rotavirus strains isolated from newborns at five hospitals in New Delhi, India, had long electropherotypes, subgroup II VP6 antigens, and G and P genotypes (G9P11) identical to those of prototype strain 116E. A novel strain with a G9P6 genotype, representing 13% of the isolates, was identified. These results demonstrate that G9P11 and G9P6 rotavirus strains are common in nurseries in New Delhi.

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Available from: Roger I Glass, Sep 10, 2014
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    • "Genotyping was done using reverse transcriptase-polymerase chain reaction (RT-PCR), to determine G-and P-types. RT- PCR was performed using a two-step amplification process as previously described [Boom et al., 1990; Gouvea et al., 1990; Gentsch et al., 1992; Das et al., 1994; Leite et al., 1996]. During the case-control study only (first year of monitoring), nucleotide sequencing was performed with strains not typed previously by RT-PCR. "
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    ABSTRACT: The monovalent human rotavirus (RV) vaccine, RIX4414 (Rotarix™, GlaxoSmithKline Biologicals) was introduced into Brazil's Expanded Program on Immunization in March 2006. One year after vaccine introduction, the G2P[4] strain was found to be predominant, with an apparent extinction of many non-G2 strains. This study investigated the diversity of circulating strains in the three years following RIX4414 introduction. Between May 2008 and May 2011, stool samples were collected from children aged ≥12 weeks who were hospitalized for severe lab confirmed RV-gastroenteritis (≥3 liquid or semi-liquid motions over a 24-h period for <14 days, requiring ≥1 overnight hospital stay and intravenous rehydration therapy) in Belém, Brazil. RV-gastroenteritis was detected by ELISA and the G- and P-types were determined by RT-PCR assays. During the first year of surveillance nucleotide sequencing was used for typing those samples not previously typed by RT-PCR. A total of 1,726 of 10,030 severe gastroentertis hospitalizations (17.2%) were due to severe RVGE. G2P[4] was detected in 57.2% of circulating strains over the whole study period, however it predominated during the first 20 months from May 2008 to January 2009. G1P[8] increased in the last part of the study period from May 2010 to May 2011 and represented 36.6% (112/306) of the circulating strains. G2P[4] was the predominant RV strain circulating during the first 20 months of the study, followed by G1P[8]. These findings probably reflect a natural fluctuation in RV strains over time, rather than a vaccine-induced selective pressure. J. Med. Virol. © 2015 Wiley Periodicals, Inc. © 2015 Wiley Periodicals, Inc.
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    • "Routine typing of the VP7 (G) and VP4 (P) antigens was carried out by multiplex semi-nested PCR targeting G1–G4, G8, G9, G10 and G12 VP7 type and P[4], P[6], P[8], P[9] P[10] and P[11] VP4 type specificities. The amplified gene fragment polymorphism was evaluated by agarose gel electrophoresis (Gentsch et al., 1992; Das et al., 1994; Ndze et al., 2013). "
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    • "K.I. Tam et al. / Infection, Genetics and Evolution 28 (2014) 524–529 525 VP2, VP3, VP6, VP7, NSP2, NSP3, NSP4, and NSP5 gene segments (Das et al., 1994; Gouvea et al., 1990; Iturriza-Gomara et al., 2001; Matthijnssens et al., 2006; Mijatovic-Rustempasic et al., 2011; Tsugawa and Hoshino, 2008 "
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    ABSTRACT: Since 2004, the Pan American Health Organization (PAHO) has carried out rotavirus surveillance in Latin America and the Caribbean. Here we report the characterization of human rotavirus with the novel G-P combination of G4P[14], detected through PAHO surveillance in Barbados. Full genome sequencing of strain RVA/Human-wt/BRB/CDC1133/2012/G4P[14] revealed that its genotype is G4-P[14]-I1-R1-C1-M1-A8-N1-T1-E1-H1. The possession of a Genogroup 1 (Wa-like) backbone distinguishes this strain from other P[14] rotavirus strains. Phylogenetic analyses suggested that this strain was likely generated by genetic reassortment between human, porcine and possibly other animal rotavirus strains and identified 7 lineages within the P[14] genotype. The results of this study reinforce the potential role of interspecies transmission in generating human rotavirus diversity through reassortment. Continued surveillance is important to determine if rotavirus vaccines will protect against strains that express the P[14] rotavirus genotype.
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