Article

T cells from the peripheral blood of coeliac disease patients recognize gluten antigens when presented by HLA-DR, -DQ, or -DP molecules.

Institute of Transplantation Immunology, National Hospital, Oslo, Norway.
Scandinavian Journal of Immunology (impact factor: 2.23). 07/1994; 39(6):567-74.
Source: PubMed

ABSTRACT Coeliac disease (CD) is a T-cell mediated immunological disease of the small intestine which is precipitated in susceptible individuals by ingestion of gluten. We recently reported that gliadin-specific T cells can be found in the small intestinal mucosa of CD patients, and that a preponderance of these T cells was restricted by the CD-associated DQ(alpha 1*0501,beta 1*0201) heterodimer. Here we report studies on whether the same is found for gliadin specific T cells in the peripheral blood of CD patients. T-cell responses towards gluten antigens in vitro were found for both most CD patients and healthy controls. Gluten-specific T-cell clones (TCC) were established from four CD patients. Although a large proportion of these TCC were restricted by DQ molecules, including the CD-associated DQ(alpha 1*0501,beta 1*0201) heterodimer, several were restricted instead by DR or DP molecules. Thus, gluten-derived peptides can be presented to T cells by several different HLA class-II molecules, and the preferential DQ(alpha 1*0501,beta 1*0201) restriction of gluten-specific T cells in the small intestinal mucosa of CD patients is less pronounced than for similar T cells in the peripheral blood.

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Keywords

CD patients
 
Coeliac disease
 
different HLA class-II molecules
 
DP molecules
 
DQ molecules
 
gliadin specific T cells
 
gliadin-specific T cells
 
gluten
 
gluten antigens
 
gluten-derived peptides
 
gluten-specific T cells
 
Gluten-specific T-cell clones
 
immunological disease
 
similar T cells
 
small intestinal mucosa
 
small intestine
 
T-cell
 
T-cell responses
 
TCC
 
vitro