Article

[Improvement of a method for detecting of strains of the plague microbe using polymerase chain reaction].

Genetika (impact factor: 0.44). 03/1994; 30(2):167-71. pp.167-71
Source: PubMed

ABSTRACT Three pairs of oligonucleotide primers, complementary to nucleotide sequences of Yersinia pestis plasmids (pPst, 9.5 kb; pCad, 70 kb; pFra, 95 kb), were used in polymerase chain reaction for high-sensitive specific detection of plague pathogen. Primer pairs P1,P2,C1,C2, and F1,F2 were used to amplify fragments of pla gene (plasmid pPst), yop1 gene (plasmid pCad of plague microbe), and caf1 gene (plasmid pFra), respectively. The method developed enables specific detection of strains from all natural loci in Russia and contiguous states as well as strains of oceanic origin. Sensitivity of method is 50-100 CFU/ml. Primer sequences enable to amplify gene fragments, located in three own plasmides of plague microbe, in the same reaction mixture. The method offers identification of plague microbe and determination of its virulence and epidemic significance.

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Keywords

amplify fragments
 
amplify gene fragments
 
caf1 gene
 
contiguous states
 
enables specific detection
 
epidemic significance
 
high-sensitive specific detection
 
natural loci
 
oceanic origin
 
oligonucleotide primers
 
own plasmides
 
pla gene
 
plague microbe
 
plague pathogen
 
plasmid pCad
 
plasmid pFra
 
plasmid pPst
 
polymerase chain reaction
 
virulence
 
Yersinia pestis plasmids
 

A N Kulichenko