Quantitative trait loci mapping of three loci controlling morphine preference using inbred mouse strains.

Department of Psychiatry and Human Behavior, Jefferson Cancer Institute, Philadelphia, Pennsylvania.
Nature Genetics (Impact Factor: 29.65). 06/1994; 7(1):54-8. DOI: 10.1038/ng0594-54
Source: PubMed

ABSTRACT Quantitative trait loci mapping was used to identify the chromosomal location of genes which contribute to oral morphine preference (in a two-bottle choice paradigm) of C57BL/6J mice, compared to DBA/2J mice. An F2 intercross of these two strains (606 mice) was phenotyped for morphine preference and those mice demonstrating extreme values for morphine consumption (the highest and lowest 7.7%) were genotyped for 157 murine microsatellite polymorphisms. Maximum likelihood methods revealed three loci on murine chromosomes 1, 6 and 10 which are responsible for nearly 85% of the genetic variance observed between the two parental strains.

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