Heterogeneity of hematopoietic stem cells

Department of Developmental Biology , Stanford University, Palo Alto, California, United States
Current Opinion in Immunology (Impact Factor: 7.48). 05/1993; 5(2):177-84. DOI: 10.1016/0952-7915(93)90002-A
Source: PubMed


Hematopoietic stem cells are capable of multi-lineage differentiation to all blood cell types as well as self-renewal and radioprotection. Thy-1.1lo Lin-/lo Sca-1+ cells are a heterogeneous mixture of quiescent and self-renewing hematopoietic stem cells as well as multi-lineage expanding cells.

2 Reads
  • Source
    • "The development of tumors in the grafts of undifferentiated iPSCs (Figure 5f, h, and j) further emphasizes the importance of developing efficient methods for differentiating iPSCs and for eliminating undifferentiated iPSCs from iPSC-KCs before transplantation. Although antibodies against cell surface markers in combination with FACS can be used for isolation of pure hematopoietic stem cell populations (Civin and Loken, 1987; Uchida et al., 1993; Bernstein et al., 1994), specific cell surface markers have not been identified for keratinocyte stem cells. As an alternative approach to enrich for iPSC-KC stem cells and to eliminate undifferentiated iPSCs, we took advantage of the previously reported ability of keratinocyte stem cells to rapidly attach to ColIV-coated surfaces (Bickenbach and Chism, 1998). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Recent breakthroughs in the generation of induced pluripotent stem cells (iPSCs) have provided a novel renewable source of cells with embryonic stem cell-like properties, which may potentially be used for gene therapy and tissue engineering. Although iPSCs have been differentiated into various cell types, iPSC-derived keratinocytes have not yet been obtained. In this study, we report the in vitro differentiation of mouse iPSCs into a keratinocyte lineage through sequential applications of retinoic acid and bone-morphogenetic protein-4 and growth on collagen IV-coated plates. We show that iPSCs can be differentiated into functional keratinocytes capable of regenerating a fully differentiated epidermis, hair follicles, and sebaceous glands in an in vivo environment. Keratinocytes derived from iPSCs displayed characteristics similar to those of primary keratinocytes with respect to gene and protein expression, as well as their ability to differentiate in vitro and to reconstitute normal skin and its appendages in an in vivo assay. At present, no effective therapeutic treatments are available for many genetic skin diseases. The development of methods for the efficient differentiation of iPSCs into a keratinocyte lineage will enable us to determine whether genetically corrected autologous iPSCs can be used to generate a permanent corrective therapy for these diseases.
    Journal of Investigative Dermatology 12/2010; 131(4):857-64. DOI:10.1038/jid.2010.364 · 7.22 Impact Factor
  • Source
    • "Many stem cell markers have been described over the past 20 years (reviewed in Visser and Bekkum, 1990, Civin and Gore, 1993, Uchida et al., 1993, Szilvassy and Hofman, 1995) . Murine HSCs are characterized by their high expression of stem cell antigen (Sca)-1 and low levels of Thy-1. "

  • Source
    • "Haematological abnormalities may also include the release of different inflammatory cytokines during the chronic phase of HIV-1 infection (Zauli et al, 1992; Esser et al, 1996). Haematopoietic progenitor cells generally are in close contact with stromal cells; these cells provide a rich milieu of cytokines, extracellular matrix proteins and adhesion molecules (Scadden et al, 1990; Uchida et al, 1993). The analysis of cytokines produced in the bone marrow (BM) may be important in the understanding of haematological abnormalities that occur in HIV-1-infected individuals. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Haematological abnormalities frequently occur in patients infected by human immunodeficiency virus-type 1 (HIV-1). Increasing evidence indicates that bone marrow suppression (BM) results from viral infection of accessory cells, with impaired stromal function and alteration of haematopoietic growth factor network. We have investigated the effects of antiretroviral therapy on cytokine and chemokine production by BM cells and stromal cells in a group of HIV-1-infected subjects before and during treatment. Compared with uninfected controls, an altered cytokine and chemokine production by BM cells was observed before treatment, characterized by decreased interleukin 2 (IL-2) and elevated tumour necrosis factor-alpha, macrophage inflammatory protein (MIP)-1alpha, MIP-1beta, and RANTES (regulated on activation, normal T cell-expressed and secreted) levels, along with a defective BM clonogenic activity. Antiretroviral therapy showed increased BM clonogenic capability, associated with normalization of IL-2 production and chemokine receptors expression on CD34+ cells. Pre-therapy, BM accessory cells were represented by macrophage-like cells, in some cases positive for HIV-1 DNA, suggesting that these cells are the main target of HIV-1 infection. During therapy, the stromal cells became predominantly fibroblastoid-like, as observed in normal controls, and were negative for HIV-1 DNA. Controlling HIV-1 replication may produce amelioration of stem cell activity, and restoration of stromal cell pattern and functions, with increased IL-2 production at BM level.
    British Journal of Haematology 10/2002; 118(3):864-74. DOI:10.1046/j.1365-2141.2002.03680.x · 4.71 Impact Factor
Show more

Similar Publications