Three-dimensional visualization of the aorta and elastic cartilage after removal of extracellular ground substance with a modified NaOH maceration method.
ABSTRACT A modified NaOH maceration method for removing extracellular ground substance was applied to scanning electron microscopic (SEM) studies of the mammalian aorta and elastic cartilage. Fixed tissues were freeze-fractured in liquid nitrogen and then immersed in 2N NaOH solution for 4-6 hr at room temperature. Since this method selectively digested proteoglycans, cellular and fibrous elements were observable clearly under the SEM. The elastic laminae of smooth muscle cells of the aortic tunica media were concealed by fine collagen fibrils, while chondrocytes in the elastic cartilage were encapsulated by a dense network of fine collagen fibrils and branching elastin fibers.
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ABSTRACT: The aim of the present study was to clarify the anatomical structure of the lamina muscularis mucosae (LMM) in the human stomach and to correlate it with the lymphatic spread of gastric cancer cells. Human stomachs taken at operation or autopsy were used. The specimens derived from these stomachs were examined by light microscopy immunohistochemistry and scanning electron microscopy (SEM). In the cardia and pyloric wall, bundles of smooth muscle cells of the LMM were relatively loose and thin and formed a reticular configuration. Small lymphatic capillaries (approximately 10-30 μm in diameter) were present directly above the LMM, and relatively large lymphatics (approximately 80-100 μm in diameter) were observed in the submucosal layer and within the LMM. In contrast, the LMM in the fundus, body, and antral wall was composed of tight, thick bundles of smooth muscle cells that ran straight. Large lymphatics were found directly beneath the LMM, but they were few in the lamina propria mucosae. In addition, lymphatics adjacent to veins were also found in the submucosa of the fundus. Structural differences in the LMM of the stomach wall might depend on physiological function. In this study, the relationship between the cytoarchitecture of the LMM or the distribution of lymphatic vessels and cancer invasion is discussed.Medical Molecular Morphology 03/2011; 44(1):39-45. · 1.17 Impact Factor
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ABSTRACT: The three-dimensional configurations of the proprial papillae in the human esophagus were observed by light microscopy, routine transmission electron microscopy, and scanning electron microscopy combined with NaOH maceration. Numerous finger-like or filiform papillae with a height of about 100m and a width at the base of approximately 30m were clearly distributed in the uppermost proprial layer at approximately equal intervals. The adepithelial surface of the proprial papillae was bordered by a reticular fiber sheet that was stained a deep black color by silver staining. The papillae possessed blood capillaries with fenestration, nerve fibers, and free cells such as lymphocytes, eosinophils, mast cells, and Langerhans-like cells. These findings clearly demonstrate characteristic three-dimensional features of proprial papillae, and their constituent cellular and structural elements in human esophagus.Medical Electron Microscopy 01/1997; 30(1):15-24.
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ABSTRACT: The posterior aspect of heel is known as a particularly vulnerable site for pressure ulcer development, however, it is not well understood why this is so. This study was undertaken to identify the morphological characteristics of the skin over posterior aspect of heel in the context of pressure ulcer development. Human skin tissues were obtained from four different sites of the body of 4 aged subjects postmortem: posterior aspect of heel, plantar aspect of heel, sacrum and centre of gluteus maximus. The skin samples were processed for the examination using light microscopy and scanning electron microscopy. The posterior aspect of heel was characterized by a thicker epidermis, denser distribution and larger diameter of capillaries in the papillary layer, 3-dimensional architecture of collagen fibre meshwork in the reticular layer and elliptic adipose tissues situated perpendicularly to the skin surface being surrounded by thick collagen and elastic fibre septa compared to the sacrum. Given our observations in the papillary layer of the posterior aspect of heel, we assume that the tissue of this area may be less tolerant to ischaemia since the tissue has high metabolic demand to provide oxygen and nutrients to the epidermis which protects underlying tissue from external force. In addition, elliptic configuration of adipose tissues in the posterior aspect of heel situated perpendicularly to the skin surface may result in deep lesion if the forces applied exceed the tolerable level since the forces will be concentrated within the elliptic compartments.Journal of tissue viability 04/2013; · 1.18 Impact Factor