Article

Antibodies to neutrophil cytoplasm in Italian patients with ulcerative colitis: sensitivity, specificity and recognition of putative antigens.

Department of Internal Medicine, University of Milan, Italy.
Digestion (impact factor: 2.05). 02/1994; 55(1):34-9. pp.34-9
Source: PubMed

ABSTRACT We studied the prevalence of perinuclear antineutrophil cytoplasmic antibody (p-ANCA), as detected by immunofluorescence, in 290 Italian subjects. One hundred and two were affected by ulcerative colitis, 48 by Crohn's disease, 40 by gluten-sensitive enteropathy and 100 were normal subjects. The prevalence of p-ANCA was significantly higher in ulcerative colitis patients (45.1%) as compared to Crohn's disease patients (4.8%), gluten-sensitive enteropathy (0%) and normal subjects (1%; p < 0.0001 ulcerative colitis vs. all other groups). In this setting, the overall specificity of the test was 98.1% with a sensitivity of 45.1%. The specificity slightly decreased to 95.1% when ulcerative colitis patients were compared to patients with Crohn's colitis. In our series, p-ANCA appeared to be more prevalent in ulcerative colitis patients with more aggressive disease. ELISA experiments performed in order to identify the putative antigen(s) recognized by p-ANCA-positive sera showed that 8 of 12 sera positive at immunofluorescence reacted with at least one of the neutrophil preparations tested. The reactivities were directed towards various neutrophil preparations. Preabsorption with the specific antigen recognized by ELISA significantly inhibited the p-ANCA immunofluorescence reactivity indicating that p-ANCA reactivity might derive from the recognition of heterogeneous neutrophil-associated antigens.

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    [show abstract] [hide abstract]
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Keywords

290 Italian subjects
 
aggressive disease
 
Crohn's disease
 
Crohn's disease patients
 
ELISA experiments
 
gluten-sensitive enteropathy
 
heterogeneous neutrophil-associated antigens
 
neutrophil preparations
 
p-ANCA immunofluorescence reactivity
 
p-ANCA reactivity
 
p-ANCA-positive sera
 
perinuclear antineutrophil cytoplasmic antibody
 
prevalence
 
putative antigen(s)
 
reactivities
 
specific antigen
 
specificity
 
ulcerative colitis
 
ulcerative colitis patients
 
various neutrophil preparations