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[Spectrophotometric determination of uric acid in serum using a titanium (IV)-porphyrin complex].

Tokyo College of Pharmacy, Japan.
Yakugaku zasshi journal of the Pharmaceutical Society of Japan (Impact Factor: 0.46). 02/1994; 114(1):48-53.
Source: PubMed

ABSTRACT Aqueous solution of oxo[5,10,15,20-tetra(4-pyridyl)porphyrinato]titanium (IV) complex, a Ti-TPyP reagent, was found to be very useful for the spectrophotometric determination of hydrogen peroxide. The reagent (lambda max 432 nm) reacts with hydrogen peroxide to form a monoperoxocomplex, resulting in a significant decrease of the absorbance at 432 nm. The decrease (delta A) in absorbance was proportional to the concentration of hydrogen peroxide. The Ti-TPyP reagent was successfully applied to the assay of uric acid in the serum, using uricase to produce hydrogen peroxide through enzymatic oxidation. Using only 5 microliters serum, a linear relationship was obtained between delta A and uric acid concentration in the serum ranging from 5 x 10(-6) to 1 x 10(-3) M. The apparent molar delta A of uric acid was 2.2 x 10(5) M-1 cm-1. The relative standard deviation of repeated runs (n = 8) was 2.8% at 3.77 x 10(-4) M uric acid. The analytical recovery of uric acid (5 x 10(-4) M) added to the serum was 96.8 to 105.0%. No pre-concentration and deproteinization were required to determine uric acid in the serum by the present method because of the high sensitivity and selectivity of the Ti-TPyP reagent for hydrogen peroxide.

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    ABSTRACT: The Ti-TPyP reagent, i.e. an acidic aqueous solution of the oxo[5,10,15,20-tetra(4-pyridyl)porphyrinato] titanium(IV) complex, TiO(tpyp), was developed as a highly sensitive and selective spectrophotometric reagent for determination of traces of hydrogen peroxide. Using this reagent, determination of hydrogen peroxide was performed by flow-injection analysis with a detection limit of 0.5 pmol per test. The method was actually applied to determination of several constituents of foods, human blood, and urine mediated by appropriate oxidase enzymes. The reaction specificity of the TiO(tpyp) complex for hydrogen peroxide was clarified from the viewpoint of the reaction mechanisms and molecular orbitals based on ab initio calculations. The results provided a well-grounded argument for determination of hydrogen peroxide using the Ti-TPyP reagent experimentally. This review deals with characterization of the high sensitivity and reaction specificity of the Ti-TPyP reagent for determination of hydrogen peroxide, to prove its reliability in analytical applications.
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