Destabilisation of lamellar dispersion of thylakoid membrane lipids by sucrose.
ABSTRACT Employing negative-staining electron microscopy, convincing evidence has been obtained for the destabilisation of multilamellar organisation of aqueous dispersions of chloroplast thylakoid membrane lipids, to an inverted micellar structure under the influence of sucrose solution. The present study provides a new insight into the phase behaviour of a naturally existing galactolipid-rich lipid mixture consequent to interaction with a kosmotropic reagent.
Full-textDOI: · Available from: Rakesh Yashroy, Aug 20, 2015
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ABSTRACT: Spinach chloroplast membranes and aqueous dispersions of their extracted lipids have been studied by spin label (stearic acid) electron spin resonance and carbon-13 nuclear magnetic resonance techniques. Combined with electron microscope studies, first systematic evidence is found for the existence of a dynamic lipid-bilayer structure in the chloroplast membranes.Journal of Biosciences 12/1990; 15(4):281-288. DOI:10.1007/BF02702669 · 1.94 Impact Factor
Conference Paper: Sample preparation for transmission electron microscopyPhysiology of Pineal gland; 01/2000
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ABSTRACT: An electron paramagnetic resonance (EPR)-based membrane surface characterization method is presented to detect the properties of the carbohydrate-rich part of membrane surfaces as well as carbohydrate interaction with other membrane constituents and water-soluble molecules. The proposed method relies on the spin-labeling and spectral decomposition based on spectral simulation and optimization with EPRSIM software. In order to increase the sensitivity of characterization to the carbohydrate-rich part of the membrane surface, the sucrose-contrasting approach is introduced. With this method, which was established on model membranes with glycolipids and tested on erythrocyte membrane, we were able to characterize the surface and lipid bilayer lateral heterogeneity. Additionally, some properties of the interaction between glycocalyx and lipid bilayer as well as between glycocalyx and sucrose molecules were determined. The experiments also provided some information about the anchoring and aggregation of the glycosylated molecules. According to the results, some functions of the glycosylated surface are discussed.Journal of Membrane Biology 06/2003; 193(1):15-22. DOI:10.1007/s00232-002-2003-7 · 2.17 Impact Factor
Questions & Answers about this publication
- How reliable is to study aqueous lipidic structures - lamellar, micellar, hexagonal phases by negative staining transmission electron microscopy? Freeze fracture techniques require samples' exposure to subzero temperatures, which is un-natural.Following
- Can galactolipid abundance in thylakoid membranes make them selectively amenable to carbohydrate generation by photosynthesis? Galctose headgroups in thylakoid membrane lipids could be a natural advantage to handling of sugars produced by photosynthesis.Following
- Can lipid hexagonal cylindrical phase be easily identified by other than freeze fracture EM e.g. by negative staining shown in paper here? Bilogicial membrane lipid aqueous structures - modes of charateristic identification.Following