Article

Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.

Department of Biochemistry, Saitama Medical School, Japan.
Proceedings of the National Academy of Sciences (Impact Factor: 9.81). 01/1994; 90(23):11117-21. DOI: 10.1073/pnas.90.23.11117
Source: PubMed

ABSTRACT Estrogen receptor (ER)-binding fragments were isolated from human genomic DNA by using a recombinant ER protein. Using one of these fragments as a probe, we have identified an estrogen-responsive gene that encodes a putative zinc finger protein. It has a RING finger motif present in a family of apparent DNA-binding proteins and is designated estrogen-responsive finger protein (efp). efp cDNA contains a consensus estrogen-responsive element at the 3' untranslated region that can act as a downstream estrogen-dependent enhancer. Moreover, efp is regulated by estrogen as demonstrated at both the mRNA and the protein level in ER-positive cells derived from mammary gland. These data suggest that efp may represent an estrogen-responsive transcription factor that mediates phenotypic expression of the diverse estrogen action. Thus, the genomic binding-site cloning may be applicable for isolation of the target genes of other transcription factors.

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