A comparative study of 1H NMR lineshape fitting analyses and biochemical lipid analyses of the lipoprotein fractions VLDL, LDL and HDL, and total human blood plasma.
ABSTRACT The purpose of this work was two-fold. In the first instance, 1H NMR spectra of the ultracentrifuged lipoprotein fractions (VLDL, LDL and HDL) from six volunteers with different clinical conditions were measured. The methylene regions of the experimental spectra were modelled in the frequency domain using non-linear lineshape fitting analyses. In this way the resolvable Lorentzian component structures of the methylene regions of these lipoprotein fraction spectra could be determined. Second, the lipoprotein fraction analyses were used to construct simplified component structures, which interpreted the lipoprotein fraction spectra well, and were feasible to use in the total plasma spectra analyses. The considerable overlap problem of the resonances was properly handled in this way. The NMR-based relative amounts of the lipoproteins (relative integrated intensities of the lipoprotein model signals) obtained were compared to the biochemically resolved relative molar percentages of the lipoprotein fractions and also of the lipid contents between the lipoprotein complexes. It was noticed that nearly all correlations were extremely good. Thus, it is suggested that the developed methodology could be used as a fast method to predict the relative amounts of the lipoproteins and also possibly the relative lipid contents between the major lipoprotein categories directly from the proton NMR spectrum of a total blood plasma sample. Furthermore, if internal or external reference for the integrated intensities of the proton NMR resonances were used, it should also be possible to obtain the absolute amounts of these quantities.
SourceAvailable from: Nana Gletsu-Miller[Show abstract] [Hide abstract]
ABSTRACT: The content of sulfur amino acid (SAA) in a meal affects postprandial plasma cysteine concentrations and the redox potential of cysteine/cystine. Because such changes can affect enzyme, transporter, and receptor activities, meal content of SAA could have unrecognized effects on metabolism during the postprandial period. This pilot study used proton NMR ((1)H-NMR) spectroscopy of human plasma to test the hypothesis that dietary SAA content changes macronutrient metabolism. Healthy participants (18-36 y, 5 males and 3 females) were equilibrated for 3 d to adequate SAA, fed chemically defined meals without SAA for 5 d (depletion), and then fed isoenergetic, isonitrogenous meals containing 56 mg·kg(-1)·d(-1) SAA for 4.5 d (repletion). On the first and last day of consuming the chemically defined meals, a morning meal containing 60% of the daily food intake was given and plasma samples were collected over an 8-h postprandial time course for characterization of metabolic changes by (1)H-NMR spectroscopy. SAA-free food increased peak intensity in the plasma (1)H-NMR spectra in the postprandial period. Orthogonal signal correction/partial least squares-discriminant analysis showed changes in signals associated with lipids, some amino acids, and lactate, with notable increases in plasma lipid signals (TG, unsaturated lipid, cholesterol). Conventional lipid analyses confirmed higher plasma TG and showed an increase in plasma concentration of the lipoprotein lipase inhibitor, apoC-III. The results show that plasma (1)H-NMR spectra can provide useful macronutrient profiling following a meal challenge protocol and that a single meal with imbalanced SAA content alters postprandial lipid metabolism.Journal of Nutrition 06/2011; 141(8):1424-31. DOI:10.3945/jn.111.138875 · 4.23 Impact Factor
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ABSTRACT: Proton MR spectra and biochemical assays have been recorded on the sera of 40 patients and ten controls in order to document the correlation between spectroscopic and biochemical variations in selected pathologies (cancer, inflammatory and infectious diseases, diabetes). N-acetyl proton resonances are essentially generated by the N-acetyl residues of the glucidic moieties borne by the most abundant acute-phase proteins (α1-acid glycoprotein, α1-antitrypsin and haptoglobin). These resonances are not correlated to immunoglobulins A, G and M levels. Principal component analysis shows that variations in spectroscopic and biochemical data are independent markers of the inflammatory status of patients but no additional sensitivity or specificity is obtained when the two sets of data are combined.Clinica Chimica Acta 02/1999; DOI:10.1016/S0009-8981(98)00166-1 · 2.76 Impact Factor
Progress in Nuclear Magnetic Resonance Spectroscopy 04/2013; 70:1-24. DOI:10.1016/j.pnmrs.2012.09.001 · 8.71 Impact Factor