Enterocyte transplantation using cell-polymer devices to create intestinal epithelial-lined tubes.

Department of Surgery, Children's Hospital, Harvard Medical School, Boston, MA 02115.
Transplantation Proceedings (Impact Factor: 0.95). 03/1993; 25(1 Pt 2):998-1001.
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    ABSTRACT: Transplantation of tissues and organs is currently the only available treatment for patients with end-stage diseases. However, its feasibility is limited by the chronic shortage of suitable donors, the need for life-long immunosuppression, and by socio-economical and religious concerns. Recently, tissue engineering has garnered interest as a means to generate cell-seeded three-dimensional scaffolds that could replace diseased organs without requiring immunosuppression. Using a regenerative approach, scaffolds made by synthetic, non-immunogenic, biocompatible materials have been developed and successfully clinically implanted. This strategy, based on a viable and ready-to-use bioengineered scaffold, able to promote novel tissue formation, favouring cell adhesion and proliferation, could become a reliable alternative to allotransplatation in the next future. In this paper, tissue engineered synthetic substitutes for tubular organs (such as trachea, esophagus, bile ducts and bowel) are reviewed, including a discussion on their morphological and functional properties.
    Journal of Biomedical Materials Research Part A 07/2014; 102(7). DOI:10.1002/jbm.a.34883 · 2.83 Impact Factor
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    ABSTRACT: Highly porous matrices of poly-L-lactide (PL) and polyglycolide (PG), 24, 50, or 95 mg/cc in the form of 10 × 10 × 3 mm wafers, were implanted subcutaneously (two per rat) in the flanks of 8–12-week-old female Lewis rats (n = 120). Matrices were harvested, two rats per week, for 15 weeks and examined histologically. At weeks 1 and 2, a thin fibrous capsule was present and matrices showed capillary beds and host-cell infiltration along the implant margins. By week 4, the PL specimens had some arterioles while the PG specimens still had only capillary beds. At week 7, PL had well developed arterioles, venules, and capillaries while PG began to show modest vascular beds of capillaries only. In terms of cellular ingrowth, PL remained unchanged from 7 to 15 weeks. Giant cell formation was observed wherever polymer was present. There was a loss of thickness and cell mass for both matrices over time (PG > PL) despite initial host-cell ingrowth. As both polymers degraded and were absorbed, the ingrown cell mass regressed. There was little remaining PG at 15 weeks, leaving no trace of cells that previously had ingrown and no evidence of scar tissue. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 41, 412–421, 1998.
    Journal of Biomedical Materials Research 09/1998; 41(3):412 - 421. DOI:10.1002/(SICI)1097-4636(19980905)41:3<412::AID-JBM11>3.0.CO;2-K
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    ABSTRACT: To describe basic principles of tissue engineering with emphasis on the potential role of gastrointestinal endoscopy in regenerative medicine. Stricturing associated with endoscopic submucosal resection and circumferential endoscopic mucosal resection can be prevented through transplantation of autologous epidermal cell sheets or seeded decellularized biological scaffolds. Lower esophageal sphincter augmentation through injection of muscle-derived cells is a novel potential treatment for gastroesophageal reflux disease. Stem cell derived tissue has been used to repair injured colon in a mouse model of colitis. A bioengineered internal anal sphincter has been successfully implanted in mice and showed preserved functionality. The immediate foreseeable application of tissue engineering in gastrointestinal endoscopy is in the field of mucosal repair after acute injury. Tissue regeneration can be achieved through expansion of autologous somatic cells or by induction of multipotent or pluripotent stem cells. Advances in cellular scaffolding have made bioengineering of complex tissues a reality. Tissue engineering in endoscopy is also being pioneered by studies looking at enteral sphincter augmentation and regeneration. The availability of engineered tissue for endoscopic application will increase with advances in cell-culturing techniques.
    Current opinion in gastroenterology 07/2013; DOI:10.1097/MOG.0b013e328363e3fd · 3.66 Impact Factor