Enterocyte transplantation using cell-polymer devices to create intestinal epithelial-lined tubes
Department of Surgery, Children's Hospital, Harvard Medical School, Boston, MA 02115.Transplantation Proceedings (Impact Factor: 0.98). 03/1993; 25(1 Pt 2):998-1001.
- Transplantation Reviews 07/1993; 7(3):153-162. DOI:10.1016/S0955-470X(05)80014-X · 3.82 Impact Factor
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ABSTRACT: Polyglycolic acid (PGA) fibre meshes are attractive candidates to transplant cells, but they are incapable of resisting significant compressional forces. To stabilize PGA meshes, atomized solutions of poly(l-lactic acid) (PLLA) and a 50/50 copolymer of poly(d,l-lactic-co-glycolic acid) (PLGA) dissolved in chloroform were sprayed over meshes formed into hollow tubes. The PLLA and PLGA coated the PGA fibres and physically bonded adjacent fibres. The pattern and extent of bonding was controlled by the concentration of polymer in the atomized solution and the total mass of polymer sprayed on the device. The compression resistance of devices increased with the extent of bonding, and PLLA bonded tubes resisted larger compressive forces than PLGA bonded tubes. Tubes bonded with PLLA degraded more slowly than devices bonded with PLGA. Implantation of PLLA bonded tubes into rats revealed that the devices maintained their structure during fibrovascular tissue ingrowth, resulting in the formation of a tubular structure with a central lumen. The potential of these devices to engineer specific tissues was exhibited by the finding that smooth muscle cells and endothelial cells seeded onto devices in vitro formed a tubular tissue with appropriate cell distribution.Biomaterials 01/1996; 17(2):115-124. DOI:10.1016/0142-9612(96)85756-5 · 8.56 Impact Factor
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ABSTRACT: The development of sufficient vascularization to maintain adequate perfusion is a primary consideration in the engineering of large tissue constructs. This research investigated the ability of aortic endothelial cells to affect the organization of vascular structures within a matrix both in vitro and in vivo. Highly porous matrices of poly(glycolic) acid (PGA) (50 mg/cc) 10 × 10 × 3 mm meshes were implanted subcutaneously (two per rat) in inbred rats, with and without syngeneic cells. Test groups (n = 8/group) were: PGA; PGA with aortic endothelial cells; PGA with aortic smooth muscle cells; PGA with skeletal muscle cells. Matrices were evaluated histologically from two rats per week at weeks 1,2,3, and 4. Scanning electron microscopy was done on matrices prior to implantation. Matrices without cells demonstrated typical ingrowth of host fibroblasts, capillaries, and macrophages/giant cells. Matrices containing skeletal muscle or aortic smooth muscle cells showed similar vascularization to matrices without cells. The implanted muscle cells demonstrated cellular growth with little organization. Matrices containing aortic endothelial cells demonstrated organized and unorganized endothelial cells within the matrix, increased numbers of capillaries, increased numbers of lymphatic-like structures, and numerous heterogeneous and unusual vascular structures which were positive for factor VIII localization including: 1) large parallel arrays of capillaries, 2) large thin sinusoidal vascular structures, and 3) layered complex vascular structures. Peer Reviewed http://deepblue.lib.umich.edu/bitstream/2027.42/63290/1/ten.1997.3.149.pdfTissue Engineering 06/1997; 3(2). DOI:10.1089/ten.1997.3.149 · 4.25 Impact Factor
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