[Show abstract][Hide abstract] ABSTRACT: The antimicrobial efficacy and anti-adhesive attributes of a proprietary denture cleanser were evaluated. To determine cleanser antimicrobial efficacy, Streptococcus mutans was grown on denture acrylic strips which were then exposed to the cleanser. To evaluate anti-adhesive efficacy, the strips were treated with the cleanser and then placed in the Strep. mutans suspension. Following incubation, adhered bacteria were removed and enumerated by viable counting. Treated denture acrylic plates were also placed in a parallel-plate flow chamber and then exposed to Strep. oralis. Images of adhered bacteria were analysed to determine biofilm coverage. Biofilm removal force was quantified by increasing the flow rate between the acrylic plates. The cleanser exhibited a 100% kill against Strep. mutans adhered to the acrylic surface, and inhibited attachment of cells by 66%. The flow chamber study found that cleanser-treated denture acrylic allowed the formation of a multilayer biofilm which was easily removed by a slight increase in flow rate.
Journal of Applied Microbiology 11/2000; 89(4):617-23. · 2.20 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Saliva, a thick, colorless, fluid that is constantly present in the humans mouth. It is composed of water, mucus, proteins, mineral salts, and amylase. As saliva circulates in the mouth cavity it picks up food debris, bacterial cells, and white blood cells. Three major pairs of salivary glands and many smaller glands scattered in the surface tissue of the cheeks, lips, tongue, and palate contribute to the total amount of saliva. Small amounts of saliva are continually being secreted into the mouth, but the presence of food, or even the mere smell or thought of it, will rapidly increase saliva flow. The functions of saliva are numerous. Primarily, it lubricates and moistens the inside of the mouth to help with speech and to change food into a liquid or semisolid mass that can be tasted and swallowed more easily. Saliva helps to control the body's water balance; if water is lacking, the salivary glands become dehydrated, leaving the mouth dry, which causes a sensation of thirst and stimulates the need to drink. Saliva reduces tooth decay and infection by removing food debris, dead cells, bacteria, and white blood cells. It also contains small amounts of the digestive enzyme amylase, which chemically breaks down carbohydrates into simpler compounds. (2).
[Show abstract][Hide abstract] ABSTRACT: Probiotics has currently attracted for means of preventive treatment measurement instead of using non-specific and broad spectrum antimicrobials. In previous studies, two main probiotics species, Lactobacillus and Bifidobateria, showed the reduction of DMFS and S. mutans counts. However, the timing of introducing probiotic species to oral cavity is not clear. The aim of this study is to evaluate the changes of binding ability of S. mutans in various concentrations and inoculation time of L. rhamnosus GG. Adding the following concentration of L. rhamnosus GG, CFU, CFU and CFU, to S. mutans medium demonstrates significant reduction of S. mutans counts. Additionally, more reduction was observed when L. rhamnosus were inoculated prior to S. mutans or simultaneously inoculated compared to when S. mutans were inoculated prior to L. rhamnosus after 3 hours of incubation. Based on this research, the timing of introducing probiotics should be considered when probiotics are utilized as a preventive treatment measurement.
THE JOURNAL OF THE KOREAN ACADEMY OF PEDTATRIC DENTISTRY. 01/2011; 38(2).
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