Article

Analysis of microsatellite instability in chronic lymphoproliferative disorders.

Laboratory of Medicine and Molecular Oncology, Department of Biomedical Sciences and Human Oncology, University of Turin, Torino, Italy.
Annals of Hematology (impact factor: 2.62). 03/1996; 72(2):67-71. pp.67-71
Source: PubMed

ABSTRACT Microsatellite instability (MSI) represents one specific pattern of genomic instability and is one of the genetic lesions most frequently detected in human neoplasia. Although MSI has been found to be associated with a wide variety of solid cancers, its involvement in lymphoid malignancies is virtually unexplored. In this study, we have investigated the presence of MSI in chronic lymphoproliferative disorders by comparing the pattern of nine microsatellite repeats (two tetranucleotides, two trinucleotides, and five dinucleotides) on autologous germline and tumor DNA of 23 patients, including 17 with B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma (B-CLL/SLL), four with hairy cell leukemia, one with lymphoplasmacytoid lymphoma, and one with T-cell chronic lymphocytic leukemia. All samples at diagnosis displayed a germline pattern of the microsatellites examined, thus suggesting that MSI is not involved in the pathogenesis of these lymphoproliferations. Also, no microsatellite alterations were observed in consecutive samples of B-CLL/SLL obtained from the same patient at various stages of the disease both before and after chemotherapy. Conversely, alterations in 3/9 microsatellite repeats were detected in one case of Richter's syndrome which had evolved from a pre-existent B-CLL/SLL phase. Overall, the low frequency of MSI among chronic lymphoproliferative disorders adds further weight to the common view that the mechanisms and patterns of genomic instability in lymphoid neoplasia differ markedly from those commonly observed in solid cancers.

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Keywords

23 patients
 
autologous germline
 
B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma
 
chronic lymphoproliferative disorders
 
common view
 
consecutive samples
 
genomic instability
 
germline pattern
 
hairy cell leukemia
 
low frequency
 
lymphoid malignancies
 
lymphoplasmacytoid lymphoma
 
microsatellite alterations
 
Microsatellite instability
 
pre-existent B-CLL/SLL phase
 
solid cancers
 
specific pattern
 
T-cell chronic lymphocytic leukemia
 
tumor DNA
 
various stages
 

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