Molecular determinants of acute inflammatory responses to biomaterials.

Division of Experimental Pathology, Albany Medical College, New York 12208, USA.
Journal of Clinical Investigation (Impact Factor: 13.77). 04/1996; 97(5):1329-34. DOI: 10.1172/JCI118549
Source: PubMed

ABSTRACT The frequent inflammatory responses to implanted medical devices are puzzling in view of the inert and nontoxic nature of most biomaterials. Because implant surfaces spontaneously adsorb host proteins, this proteinaceous film is probably important in the subsequent attraction of phagocytes. In fact, earlier we found that acute inflammatory responses to experimental polyethylene terephthalate implants in mice require the precedent adsorption of one particular host protein, fibrinogen. The present investigations were aimed at defining the molecular determinants of fibrinogen-mediated acute inflammatory responses to implanted biomaterials. We find: (a) plasmin degradation of purified fibrinogen into defined domains reveals that the proinflammatory activity resides within the D fragment, which contains neither the fibrin cross-linking sites nor RGD sequences; (b) the major (and, perhaps, exclusive) proinflammatory sequence appears to be fibrinogen gamma 190-202, previously shown to interact with CD11b/CD18 (Mac-1). The chemically synthesized peptide, cross-linked to albumin (which itself does not promote inflammatory responses), mimics the proinflammatory effect of adsorbed native fibrinogen; and (c) this sequence probably promotes inflammatory responses through interactions with Mac-1 because phagocyte accumulation on experimental implants is almost completely abrogated by administration of recombinant neutrophil inhibitory factor (which blocks CD11b-fibrin(ogen) interaction). We conclude that improved knowledge of such surface-protein-phagocyte interactions may permit the future development of more biocompatible implantable materials.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Abdominal aorta aneurysm (AAA) is a serious threat for human life. AAA repair is a high-risk procedure which results in a severe surgical stress response. We aim to give a conceptual description of the underlying pathophysiology of stress after surgical repair of AAA.
    International Journal of Clinical and Experimental Medicine 01/2014; 7(9):2402-12. · 1.42 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Interaction of host cells with a biomaterial surface is important for biocompatibility and thus is essential for biomedical applications. Therefore investigations are undertaken to scrutinize for an appropriate surface coating with physical and chemical properties minimizing undesirable activation of immunological response. For this the current study was aimed at examining the effects of different surface modifications of titanium by its coating with ceramic materials - hydroxyapatite, bioglass and CaO-SiO2 on osteoblast morphology and secretory activity. Titanium is known for its excellent mechanical properties but its surface has low bioactivity. We report that CaO-SiO2 coating decreased a number of attached osteoblasts and altered their morphology. Moreover, the ceramic coatings temporarily upregulated release of pro-inflammatory cytokines IL-6 (all of them) and TNF-α (CaO-SiO2). However, overall the levels of the cytokines were low. In contrast, levels of neutrophil-attracting chemokine IL-8 were the highest. IL-8 was produced mostly by cells incubated with hydroxyapatite titanium coating in contrary to those incubated with either bioglass or CaO-SiO2 titanium modifications. In conclusion, the titanium coated with ceramics such as hydroxyapatite or bioglass had the best effect on cell adhesion; however, hydroxyapatite might potentially stimulate destructive neutrophils while CaO-SiO2-coating has a negative effect on cell adhesion.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The presented work demonstrates preliminary results on manufacturing and mechanical properties assessment of pure PLLA and PLLA/TCP composite plates, as potential candidates for maxillofacial surgery application. Biodegradable, thermoplastic polymer poly-L-lactide (PLLA, Biomer) and tricalcium phosphate (TCP, Fluka) particles were applied for the samples manufacturing in injection moulding process. The plates were investigated using universal testing machine Zwick 1435 – in static tensile and creep tests. The mechanical properties (tensile strength, Young modulus, elongation) of the polymer and its composite were determined and the lifetime, performed with the scope of their potential application in osteosynthesis, were predicted.

Full-text (2 Sources)

Available from
Jun 2, 2014