The prognosis of oral mucosal squamous cell carcinomas: a comparison of clinical and histopathological grading and of laminin and type IV collagen staining.
ABSTRACT Changes in the distribution of basement membrane components have been described in dysplastic lesions and in oral mucosal squamous cell carcinomas (OMSCC). The purpose of this study was to determine if these changes were related to pathological grade and if so, whether this had prognostic implications. Fifty formalin-fixed, paraffin-embedded specimens of OMSCC, with five or more years clinical follow-up, were studied using an immunoperoxidase technique for the detection of the basement membrane components, laminin and type IV collagen. The immunoreactivity of each component was evaluated and semiquantitatively scored as minimal, moderate or extensive and the results compared with the tumour size, node involvement and metastasis (TNM) clinical staging system and histopathological features. OMSCC were characterized by minimal or moderate staining with small islands of neoplastic cells frequently lacking staining for laminin and type IV collagen. Deposition of these components decreased with increased histopathological grade and absence of staining was more commonly associated with a poor prognosis. In particular the pattern of type IV collagen staining frequently differed from laminin staining. Neither of these parameters offered an advantage over TNM clinical staging with regard to prognosis. It was concluded that variations in laminin and type IV collagen immunoreactivity occurred in OMSCC and that high histopathological grade tumours with considerably diminished staining with anti-laminin and anti-type IV collagen carried a poor prognosis.
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ABSTRACT: During nephrogenesis, transition of mesenchyme to the epithelium of tubules and glomeruli occurs via the interaction of ureteral bud and metanephric mesenchyme. The distribution pattern of collagen type IV suggests that a regulated balance of activities is required to facilitate migration of the ureteral bud branches into the mesenchyme and to control early extracellular matrix changes during tubulogenesis. We used a specific antibody for tracing collagen type IV basement membrane during renal tubules morphogenesis. Twenty female Balb/C mice were divided randomly into 10 groups and were kept until finding vaginal plug was as an indicator of day zero of pregnancy. Twelve pregnant mice were sacrified by cervical dislocation in one of gestational days 13 to 18 and their fetuses were fixed, serially sectioned, and underwent immunohistochemical study for tracing of collagen type IV in basement membrane of glomeruli. The same processes were used for kidneys preparation on postnatal days 5, 10, 15, and 20 in newborns of 2 mothers for each day. Collagen type IV showed weak reaction on day 14 of gestation in tubular basement membrane. The amount of collagen increased continuously until the following days of fetal life and of the first 5 postnatal days in basement membrane. After this period, collagen type IV reaction was not showed significant change in newborns. These results indicate that developmental changes in various nephron segments from most immature stages to most differentiated structures are dependent on the collagen type IV expression.Urology journal 01/2009; 6(4):289-94. · 0.56 Impact Factor
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ABSTRACT: To determine the immunohistochemical localization of basement membrane components laminin and type IV collagen in premalignant and malignant lesions of the oral epithelium. Formalin-fixed tissue sections of 12 epithelial hyperplasias with no dysplasia and 30 dysplasias, clinically diagnosed as leukoplakia and/or erythroplakia, as well as 50 invasive squamous cell carcinomas, were stained with mouse monoclonal antibodies to human laminin and type IV collagen. Statistical analysis showed that there was a linear trend for discontinuous distribution of laminin from epithelial hyperplasia to epithelial dysplasia and invasive squamous cell carcinoma (P < 0.001). Laminin staining showed a linear trend for discontinuity with increasing grade of dysplasia (P < 0.05) and was more frequently discontinuous in areas of deep tumour invasion than in central or superficial areas (P < 0.05). Brush-shaped thickening and reduplication of the basement membrane were also identified. Alterations in the distribution of laminin and type IV collagen in oral premalignant and malignant lesions indicate that the loss of continuity of the subepithelial basement membrane parallels the progression of the neoplastic transformation process in oral epithelium.Histopathology 09/1998; 33(3):261-8. · 2.86 Impact Factor
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ABSTRACT: Invasion and metastasis are two characteristics of malignant tumors, which perform by proteolytic destruction of the components of basement membrane (BM) and cell migration. The aim of this study was to evaluate the immunohistochemical (IHC) assessment of type IV collagen and laminin-332 γ2 (Ln-332 γ2) chain expression in well-differentiated oral squamous cell carcinoma (OSCC) and oral verrucous carcinoma (OVC), because these two lesions have same histopathologic findings whereas they have different biological behaviors. Destruction of BM and cell migration were evaluated by IHC in 15 cases of epithelial hyperplasia with no dysplasia (A group), 15 cases of OVC (B group) and 15 cases of well-differentiated OSCC (C group). There was a significant difference in type IV collagen immunohistochemical staining between three groups, but there were no significant differences between B and C groups. Expression of Ln-332 γ2 chain was not detected in A group. Ln-332 γ2 chain labeling index had significantly difference between B and C groups. The number of Ln-332 γ2 chain immunostaining positive cells was less than 5% in B group and over than 5% in C group which there were significantly differences between these two groups. Isolated immunohistochemical study of type IV collagen does not clearly define that a lesion is invasive or non-invasive and evaluation of Ln-332 γ2 chain expression (cut-off 5%) may be useful as a marker for description of biological differences and diagnosis of OVC from well-differentiated OSCC, especially in doubtful cases.Journal of Oral Pathology and Medicine 02/2011; 40(2):167-73. · 2.06 Impact Factor