Article

Structural analysis of the mouse activin beta C gene.

Department of Pathology, Baylor College of Medicine, Houston, TX 77030, USA.
Biochimica et Biophysica Acta (impact factor: 4.66). 07/1996; 1307(2):145-8. pp.145-8
Source: PubMed

ABSTRACT Using a mouse activin beta C cDNA probe, we have cloned over 38 kb of genomic DNA encompassing the mouse activin beta C gene. Mouse activin beta C is encoded by 2 exons separated by a large 12.1 kb intron. Exon 1 encodes the 17 amino acid signal peptide and 88 amino acids of the propeptide. Exon 2 encodes the remaining 130 amino acids of the propeptide and the entire 117 amino acid mature peptide. Unlike the activin beta A and beta B expression patterns, Northern blot analysis of adult mouse tissues shows that the activin beta C mRNA is expressed only in the liver as a major species of approximately 2.1 kb. The liver-specific expression of activin beta C suggests an important role of dimeric activin beta C in normal liver function. These studies allow us to address the function of activin beta C in mammalian development.

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Keywords

17 amino acid signal peptide
 
2 exons
 
88 amino acids
 
activin beta
 
activin beta C
 
activin beta C mRNA
 
adult mouse tissues
 
dimeric activin beta C
 
entire 117 amino acid mature peptide
 
genomic DNA encompassing
 
large 12.1 kb intron
 
liver-specific expression
 
mammalian development
 
Mouse activin beta C
 
mouse activin beta C cDNA probe
 
mouse activin beta C gene
 
normal liver function
 
Northern blot analysis
 
propeptide
 

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