Characterization of a novel anti-inflammatory factor produced by RM3/1 macrophages derived from glucocorticoid treated human monocytes.
ABSTRACT Glucocorticoids exert their anti-inflammatory activity by modulating the functions of various cell types including macrophages. They also induce the generation of a distinct macrophage subtype defined by the surface antigen RM3/1 which appears to be associated with the down-regulation of inflammation. Supernatants from these cells were found to exert a dose-dependent anti-inflammatory effect, particularly in the early phase as shown in the 5-hydroxytryptamine (5-HT) induced footpad edema of mice. By using conventional purification methods the anti-inflammatory factor was found to have a molecular mass of about 78 kD and an isoelectric point of about 7.9. Heat lability and sensitivity to trypsin and proteinase K indicate the protein nature of the anti-inflammatory factor. The inhibition of the early phase of inflammation and the molecular weight suggest that the anti-inflammatory agent released from RM3/1 macrophages is a novel protein different from other anti-inflammatory proteins described so far.
- SourceAvailable from: ncbi.nlm.nih.govBritish Journal of Pharmacology 09/1988; 94(4):987-1015. · 5.07 Impact Factor
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ABSTRACT: Leukocyte cell concentrates, obtained by continuous flow leukapheresis from single donors, were separated on a continuous hypotonic (260 mosM) Percoll gradient. On average, 86% of monocytes were recovered in a sharp band at a purity of up to 91% (average 76%). By this procedure 1-2 X 10(9) monocytes may be obtained from an individual donor. Hypotonic gradient purification, as compared with isotonic (295 mosM) conditions, proved superior with regard to capacity, speed of performance, yield and monocyte purity.Journal of Immunological Methods 12/1982; 54(3):309-15. · 2.23 Impact Factor
- Biochimica et Biophysica Acta 05/1994; 1197(1):63-93. · 4.66 Impact Factor