Article

High-frequency endonuclease (REA) typing: results from the WHO collaborative study group on subtyping of Listeria monocytogenes.

Department of Clinical Microbiology, Statens Seruminstitut, Copenhagen S, Denmark.
International Journal of Food Microbiology (impact factor: 3.33). 11/1996; 32(3):313-24. pp.313-24
Source: PubMed

ABSTRACT Eighty isolates of Listeria monocytogenes were typed by high-frequency restriction endonuclease analysis. Two laboratories participated in the study with two restriction enzymes each. The enzymes used were EcoRI, Hae1II, HhaI. and its isoschizomer Cfo1. EcoRI was less discriminatory than the other enzymes. The profiles generated by Hha1 and CfoI were not fully stable for some closely related isolates. The size and the number of restriction bands generated by Hha1 in one laboratory and its isoschizomer Cfo1 in another laboratory were directly comparable. This indicates that REA-typing may be used as a definitive typing method for Listeria monocytogenes if the typing procedure is standardized. The stability of the REA-types needs further elucidation in order to establish firm differentiation criteria for comparison of isolates.

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Keywords

CfoI
 
definitive typing method
 
EcoRI
 
enzymes
 
firm differentiation criteria
 
HhaI
 
high-frequency restriction endonuclease analysis
 
isoschizomer Cfo1
 
Listeria monocytogenes
 
REA-types
 
REA-typing
 
restriction bands
 
restriction enzymes
 
typing procedure
 

P Gerner-Smidt