Article

Expression patterns of Id1, Id2, and Id3 are highly related but distinct from that of Id4 during mouse embryogenesis.

Cell Biology Program, Memorial Sloan Kettering Cancer Center, New York, New York 10021, USA.
Developmental Dynamics (impact factor: 2.54). 12/1996; 207(3):235-52. DOI:10.1002/(SICI)1097-0177(199611)207:3<235::AID-AJA1>3.0.CO;2-I pp.235-52
Source: PubMed

ABSTRACT The murine dominant negative helix-loop-helix (dnHLH) proteins inhibit the activities of bHLH transcription factors in diverse cell lineages (Benezra et al. [1990] Cell 61:49-59; Christy et al [1991] Proc. Natl. Acad. Sci. U.S.A. 88:1815-1819; Sun et al [1991] Mol. Cell Biol. 11: 5603-5611; Riechmann et al. [1994] Nucleic Acids Res. 22:749-755). Currently, there are four members in the dnHLH family, Id1, Id2, Id3, and Id4. In this report, we have performed a detailed comparative in situ hybridization analysis to examine their expression pattern during post-gastrulational mouse development. Id1, 2, and 3 are expressed in multiple tissues, whereas Id4 expression can only be detected in neuronal tissues and in the ventral portion of the epithelium of the developing stomach. The regions where Id1-3 genes are expressed, such as gut, lung, kidney, tooth, whisker, and several glandular structures, are undergoing active morphogenetic activities. The expression patterns of Id1, 2, and 3 overlap in many organs, except in the tissue derived from primitive gut. In the latter, Id1 and Id3 signals are detected in the mesenchyme surrounding the epithelium, whereas Id2 is expressed within the epithelium. The difference in the patterns of expressions of Id2-3 and Id4 suggest that the dominant negative transcriptional activity of these two subclasses of the Id family may have different physiological consequences.

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    Scott A Gahr, M Fernanda Rodriguez, Caird E Rexroad
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    Carolin Manthey, Demissew S Mern, Anja Gutmann, Anne J Zielinski, Corinna Herz, Silke Lassmann, Jens Hasskarl
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    BMC Cell Biology 01/2010; 11:2. · 2.59 Impact Factor
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    Anna Lasorella, Gerson Rothschild, Yoshifumi Yokota, Robert G Russell, Antonio Iavarone
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    Molecular and Cellular Biology 06/2005; 25(9):3563-74. · 5.53 Impact Factor

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Keywords

bHLH transcription factors
 
Cell Biol
 
detailed comparative
 
developing stomach
 
diverse cell lineages
 
dominant negative transcriptional activity
 
expression pattern
 
expression patterns
 
glandular structures
 
Id1-3 genes
 
Id3 signals
 
Id4 expression
 
multiple tissues
 
murine dominant negative helix-loop-helix
 
neuronal tissues
 
Nucleic Acids Res
 
post-gastrulational mouse development
 
situ hybridization analysis
 
undergoing active morphogenetic activities
 
ventral portion
 

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