Transplantation studies have demonstrated that glia-depleted areas of the CNS can be reconstituted by the introduction of cultured cells. Thus, the influx of Schwann cells into glia-free areas of demyelination in the spinal cord can be prevented by the combined introduction of astrocytes and cells of the O-2A lineage. Although Schwann cell invasion of areas of demyelination is associated with destruction of astrocytes, the transplantation of rat tissue culture astrocytes ("type-1") alone cannot suppress this invasion, indicating a role for cells of the O-2A lineage in reconstruction of glial environments. By transplanting different glial cell preparations and manipulating lesions so as to prevent meningeal cell and Schwann cell proliferation it is possible to demonstrate that the behaviour of tissue culture astrocytes ("type-1") and astrocytes derived from O-2A progenitor cells ("type-2") is different. In the presence of meningeal cells, tissue culture astrocytes clump together to form cords of cells. In contrast, type-2 astrocytes spread throughout glia-free areas in a manner unaffected by the presence of meningeal cells or Schwann cells. Thus, progenitor-derived astrocytes show a greater ability to fill glia-free areas than tissue culture astrocytes. Similarly, when introduced into infarcted white matter in the spinal cord, progenitor-derived astrocytes fill the malacic area more effectively than tissue culture astrocytes, although axons do not regenerate into the reconstituted area.
[Show abstract][Hide abstract] ABSTRACT: [EN]Development. Brain and spinal cord lesions have an increasing social and economic importance. Accidental trauma of various kinds is the main cause of mortality of children and young adults in developed countries. Only cardiac disease and cancer surpass the number of death caused by accidents and, examining the number of potential work-years lost, CNS lesions surpass all other problems. Most brain and spinal cord injuries cause chronic incapacity and frequently occur to individuals under 45 years of age. Edema and other acute events can be efficiently treated and CNS lesions may not be mortal, but are incurable. Conclusion. The final outcome of CNS injury depend on the area damaged and the extent of the lesion, but the best present therapies can offer is relief of the symptoms and rehabilitation. This review examines the present state of functional repair of experimental central nervous system trauma. [ES]Desarrollo. Las lesiones de cerebro y médula espinal están adquiriendo una creciente importancia social y económica. En los países desarrollados, el trauma accidental es la causa principal de la muerte de niños y adultos jóvenes. Solamente las enfermedades cardíacas y el cáncer superan a los accidentes como causa de mortalidad y, si examinamos los años de trabajo potencial perdidos, las lesiones del sistema nervioso central (SNC) superan a todos los demás problemas. La mayoría de las lesiones de cerebro y médula espinal ocurren en individuos menores de 45 años de edad y causan incapacidad crónica. El edema y otros fenómenos de fase aguda pueden tratarse eficazmente y las lesiones del SNC no son mortales, pero sí incurables. Conclusión. Las consecuencias finales de una lesión del SNC dependen del lugar dañado y la magnitud de la lesión; lo mejor que las terapias actuales pueden ofrecer es alivio de los síntomas y rehabilitación. Esta revisión examina el estado actual de la reparación funcional de lesiones experimentales traumáticas del sistema nervioso central. El trabajo de nuestro equipo ha sido financiado por el CSIC, el FIS, la CICYT y la Unión Europea. Peer reviewed
[Show abstract][Hide abstract] ABSTRACT: Twenty-one volunteers were exposed in a radon chamber during well-controlled aerosol and radon progeny conditions, with predominantly unattached radon daughters (Po-218, Pb-214 and Bi-214). The activity of these radionuclides deposited in the respiratory tract was measured in-vivo after the exposures. The results of these measurements are in agreement with predictions calculated with the ICRP Publication 66 Human Respiratory Tract Model (HRTM). Temporal analysis of the activity deposited in the head of the volunteers leads to the conclusion that a significant amount of the deposited activity associated with particle diameters of about 1 nm is not subject to fast particle transport to the gastrointestinal tract as generally reported for larger aerosol particles. Measurements of radon progeny in blood samples of these volunteers yielded absorption half-times of 20 to 60 min. Former determinations, mainly performed with much larger aerosol particles of diameters between 100 and 1000 nm, derived absorption half-times around 10 h. This indicates that the absorption from ciliated airways into blood is dependent upon particle size and particle composition.
Radioactivity in the Environment 01/2005; 7. DOI:10.1016/S1569-4860(04)07035-4
[Show abstract][Hide abstract] ABSTRACT: The clinical prospect of using neural precursor cells for reconstructive approaches in the nervous system has received strong impetus from a recent series of important experimental findings. Transplantation studies in the developing brain have demonstrated that migration and differentiation of neural precursor cells are regulated predominantly by environmental signals. Several observations suggest that the mature CNS retains at least some of these guidance cues. These findings, together with recent evidence for the persistence of neural stem cells in the adult mammalian brain, have made precursor cell recruitment a new focus in CNS reconstruction.
Current Opinion in Neurobiology 11/1996; 6(5):688-95. DOI:10.1016/S0959-4388(96)80104-8 · 6.63 Impact Factor
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