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Monoclonal Antibodies Against Streptococcus Pneumoniae Detect Epitopes on Eubacterial Ribosomal Proteins L7/L12 and on Streptococcal Elongation Factor Ts

National Institute of Public Health, Department of Vaccinology, Oslo, Norway.
Microbiology (Impact Factor: 2.84). 02/1997; 143 ( Pt 1)(1):55-61. DOI: 10.1099/00221287-143-1-55
Source: PubMed

ABSTRACT Two monoclonal antibodies (mAbs) designated 144,H-3 (IgG2a) and 218,C-5 (IgM) were produced after immunization of mice with two different heat-treated and sonicated pneumococcal strains. Western blotting, with solubilized proteins from different bacterial genera and from mammalian lymphocytes, showed that both mAbs reacted with a protein of approximately 12 kDa in all 66 strains of eubacteria examined, representing 27 different species. The 12 kDa protein was isolated by immunoaffinity chromatography. Subsequent preparative Western blotting enabled N-terminal amino acid sequence analysis by microsequencing. A high degree of amino acid sequence similarity with eubacterial ribosomal proteins L7/L12 was demonstrated. One of the mAbs (144,H-3) also cross-reacted in Western blotting with a 43 kDa protein, but only from streptococci. The 43 kDa protein carrying the common streptococcal epitope was isolated and sequenced in the N-terminal region. A high degree of amino acid sequence identity was found to elongation factor Ts from Escherichia coli.

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    • "Published by Elsevier Science B.V. All rights reserved. PII: S 0 9 2 8 -8 2 4 4 ( 0 1 ) 0 0 2 5 6 -5 hyde-3-phosphate dehydrogenase, ribosomal protein L7/ L12 and elongation factor Ts [6] [7] "
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