Purification, structure and activity of three insect toxins from Buthus occitanus tunetanus venom

Laboratoire des Venins et Toxines, Institut Pasteur de Tunis, Belvédère, Tunisia.
Toxicon (Impact Factor: 2.58). 04/1997; 35(3):365-82. DOI: 10.1016/S0041-0101(96)00173-0
Source: PubMed

ABSTRACT One contractive and two depressant toxins active on insect were purified by high-performance liquid chromatography from the venom of Buthus occitanus tunetanus (Bot). The two depressant toxins, BotIT4 and BotIT5, differ only at position 6 (Arg for Lys) and are equally toxic to insects (LD50 to Blatella germanica = 110 ng/100 mg body weight). They show a strong antigenic cross-reaction with a depressive toxin from Leiurus quinquestriatus quinquestriatus (LqqIT2). The two toxins are able to inhibit with high affinity (K0.5 between 2 and 3 nM) the specific binding of the radioiodinated excitatory insect toxin (125I-AaHIT) on its receptor site on Periplaneta americana synaptosomal membranes. These toxins depolarize the cockroach axon, irreversibly block the action potential, and slow down and very progressively block the transmembrane transient Na+ current. The contracturant toxin BotIT1 is highly toxic to B. germanica (LD50 = 60 ng/ 100 mg body weight) and barely toxic to mice (LD50 = 1 microgram/20 g body weight) when injected intracerebroventricularly. It does not compete with 125I-AaHIT for its receptor site on P. americana synaptosomal membranes. On cockroach axon, BotIT1 develops plateau potentials and slows down the inactivation mechanism of the Na+ channels. Thus, BotIT1 belongs to the group of alpha insect-selective toxins and shows a strong sequence identity (> 90%) with Lqh alpha IT and LqqIII, two insect alpha-toxins previously purified from the venom of L. q. hebraeus and L. q. quinquestriatus. respectively.

1 Follower
  • Source
    • "(2) Anti-insect a-NaScTXs, that are highly active only on insect VGSCs. Examples of these toxins are: LqhaIT (Eitan et al., 1990), Lqq3 (Kopeyan et al., 1993), and BotIT1 (Borchani et al., 1997), which bind with high affinity to insect neuronal preparations (0.06–1 nM). (3) a-Like toxins, active on both insect and mammalian "
    [Show abstract] [Hide abstract]
    ABSTRACT: The number and types of venom components that affect ion-channel function are reviewed. These are the most important venom components responsible for human intoxication, deserving medical attention, often requiring the use of specific anti-venoms. Special emphasis is given to peptides that recognize Na(+)-, K(+)- and Ca(++)-channels of excitable cells. Knowledge generated by direct isolation of peptides from venom and components deduced from cloned genes, whose amino acid sequences are deposited into databanks are now adays in the order of 1.5 thousands, out of an estimate biodiversity closed to 300,000. Here the diversity of components is briefly reviewed with mention to specific references. Structural characteristic are discussed with examples taken from published work. The principal mechanisms of action of the three different types of peptides are also reviewed. Na(+)-channel specific venom components usually are modifier of the open and closing kinetic mechanisms of the ion-channels, whereas peptides affecting K(+)-channels are normally pore blocking agents. The Ryanodine Ca(++)-channel specific peptides are known for causing sub-conducting stages of the channels conductance and some were shown to be able to internalize penetrating inside the muscle cells.
    Toxicon 07/2013; 76. DOI:10.1016/j.toxicon.2013.07.012 · 2.58 Impact Factor
  • Source
    • "The synaptosomal preparation is easy to obtain and has been applied in several pharmacological tests. As illustration, binding assays of many radiolabeled toxins have been successfully characterized with synaptosomes [24] [42] [43] [68]. In addition, studies of photoaffinity labeling using 125 I TsVII as a ligand in synaptosomes of nerve cord from cockroach indicated for the first time the molecular weight of the scorpion toxin receptor from the insect nervous system which was suggested to be associated with voltage sensitive Na + channels [68]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Nervous system of Periplaneta americana cockroach is used in a wide range of pharmacological studies, including electrophysiological techniques. This paper presents its role as a preparation in the development of toxinological studies in the following electrophysiological methods: double-oil-gap technique on isolated giant axon, patch-clamp on DUM (dorsal unpaired median) neurons, microelectrode technique in situ conditions on axon in connective and DUM neurons in ganglion, and single-fiber oil-gap technique on last abdominal ganglion synapse. At the end the application of cockroach synaptosomal preparation is mentioned.
    Journal of Toxicology 05/2012; 2012:143740. DOI:10.1155/2012/143740
  • Source
    • "The long-chain insect toxins can be further subdivided into two groups: the depressant toxins and the excitatory toxins[6]. The former induces a progressive flaccid paralysis of insects by blocking the action potentials[7], and is represented by BmK IT4, Aa IT5, Ba IT2, Bot IT4 and Bot IT5, Bj IT2, Lqh IT2, and Lqq IT2[1], [8] [9] [10] [11] [12] [13]. The later causes an immediate spastic paralysis of insects by inducing repetitive firing in the motor nerves[14], and is represented by AaIT, Bj IT1, Bjxtr IT, Lqq IT1, Amm IT, Lqh IT1 [12],[13], [15] [16] [17]. "
    [Show abstract] [Hide abstract]
    ABSTRACT: The nucleotide sequence deduced from the amino acid sequence of the scorpion insectotoxin AaIT was chemically synthesized and was expressed in Escherichia coli. The authenticity of this in vitro expressed peptide was confirmed by N-terminal peptide sequencing. Two groups of bioassays, artificial diet incorporation assay and contact insecticidal effect assay, were carried out separately to verify the toxicity of this recombinant toxin. At the end of a 24 h experimental period, more than 60% of the testing diamondback moth (Plutella xylostella) larvae were killed in both groups with LC50 value of 18.4 microM and 0.70 microM respectively. Cytotoxicity assay using cultured Sf9 insect cells and MCF-7 human cells demonstrated that the toxin AaIT had specific toxicity against insect cells but not human cells. Only 0.13 microM recombinant toxin was needed to kill 50% of cultured insect cells while as much as 1.3 microM toxin had absolutely no effect on human cells. Insect cells produced obvious intrusions from their plasma membrane before broken up. We infer that toxin AaIT bind to a putative sodium channel in these insect cells and open the channel persistently, which would result in Na+ influx and finally cause destruction of insect cells.
    Cell Research 07/2002; 12(2):143-50. DOI:10.1038/ · 11.98 Impact Factor
Show more