CD8Tc1 and Tc2 cells secrete distinct cytokine patterns in vitro and in vivo but induce similar inflammatory reactions.
ABSTRACT Naive CD8 T cells, similar to CD4 T cells, can differentiate into at least two subsets of cytolytic effector cells with distinct cytokine patterns: T cytotoxic-1 (Tc1) cells secrete a Th1-like cytokine pattern, including IL-2 and IFN-gamma; and Tc2 cells produce Th2 cytokines, including IL-4, IL-5, and IL-10. As CD4 Th1 cells induce delayed-type hypersensitivity (DTH) more effectively than Th2 cells, we tested the potential ability of Tc1 and Tc2 cells to induce DTH. Allospecific Tc1 or Tc2 cells were injected into the footpads of naive mice expressing the target Ag. Tc1 and Tc2 cells induced comparable levels of Ag-specific footpad swelling with similar kinetics. They also induced similar levels of footpad edema and similar infiltration of macrophages and neutrophils. However, Tc2 cells induced slightly more eosinophil infiltration. Analysis of footpad extracts showed that Tc1 and Tc2 cells retained their distinct in vitro cytokine profiles in the injected footpads. These results suggest that both Tc1 and Tc2 cytokines can be associated with the DTH reaction induced by CD8 T cells. Perforin-deficient Tc1 or Tc2 cells also induced DTH, although at lower levels, suggesting that perforin-mediated cytotoxicity of CD8 T cells is not essential for CD8-induced DTH. Thus, despite their distinct cytokine profiles in vitro and in vivo, Tc1 and Tc2 cells induce similar DTH reactions.
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ABSTRACT: CD8(+) T cells have long been suggested to play a role in rheumatoid arthritis (RA). The current paradigm on the pathogenesis and maintenance of the disease would endorse these cells with predominantly protective and minor influences. However, several animal studies suggest that these cells may have a predominantly proinflammatory (cytotoxic) effect in the disease. Other studies claim otherwise, that they have a mainly regulatory role in inflammatory joints. The evidence in human disease is remarkably scarce. Studies in human samples indicate that CD8(+) T cells play an important role in the establishment of germinal centers observed in nearly 50% of RA patients, which may have a decisive role in the initiation and maintenance of the disease process. The conflicting results of experimental studies, the scarcity of data and the complexity of research needed to unravel these complex interactions may explain the relative oblivion of CD8 cells in the field of arthritis over recent decades. Is this a wise decision or may we run the risk of not finding the key to RA because we search for it where there is light as opposed to its probable location? The present review brings together available data on the potential role of CD8(+) T cells in inflammation, with emphasis on rheumatoid arthritis, hoping to foster interest and fresh research in this area.Autoimmunity reviews 07/2012; 12(3). DOI:10.1016/j.autrev.2012.07.011 · 7.10 Impact Factor
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ABSTRACT: Background and objectivesDetailed information on CD8+ T cells in rheumatoid arthritis (RA) is still reduced. However, studies on animal models of arthritis from the authors' team and others suggest a major potential role of CD8+ T cells in the pathogenesis of chronic polyarthritis.In the present study the authors characterised the phenotype and cytokine-production profile of CD8+ T cells from peripheral blood (PB) and synovial fluid (SF) of RA patients.Materials and methodsUnstimulated CD8+ T cells from the PB and SF of 40 patients with established RA were analysed by flow-cytometry for cell surface markers expression and intracellular cytokine production, and compared to the ones present in the PB of 15 healthy donors.Results40% of the total T cells infiltrating the SF were CD8+. The SF was characterised by a significant (pAnnals of the Rheumatic Diseases 02/2011; 70(2). DOI:10.1136/ard.2010.148981.10 · 9.27 Impact Factor
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ABSTRACT: The eosinophil cell has been related as a prognostic indicator for cancers. However, its exact function in tumour behaviour is still not clearly defined. In the oral cavity the presence of eosinophils can be a favourable prognostic indicator as well as it may be associated with a poor prognosis. In this short review, we briefly summarize the role of the eosinophils in the general context of immunoregulation and its relation to oral squamous cell carcinoma.Journal of Oncology 12/2009; 2009:310132. DOI:10.1155/2009/310132