Article

Eosinophil cationic protein and histamine after intestinal challenge in patients with cow's milk intolerance.

Asthma and Allergy Research Center, Sahlgrens' Hospital, Göteborg, Sweden.
Journal of Allergy and Clinical Immunology (Impact Factor: 12.05). 09/1997; 100(2):216-21. DOI: 10.1016/S0091-6749(97)70227-6
Source: PubMed

ABSTRACT Mast cells and eosinophils are key cells in the development of active symptoms in allergic diseases and other inflammatory conditions, and they mediate their action through the release of very potent granule constituents.
Five patients with milk-related gastrointestinal symptoms diagnosed by double-blind placebo-controlled milk challenges, but with negative responses to skin prick tests and RASTs with milk, and eight healthy control subjects were investigated. Repeated perfusion studies were performed with a two-balloon, six-channel tube by using milk, casein, and whey as antigens. Luminal eosinophil cationic protein, histamine, and albumin were measured by radioimmunoassay.
Luminal cow's milk induced a pronounced increase in intestinal secretion of histamine and eosinophil cationic protein in patients, but not control subjects, during the first 20 minutes after challenge (histamine from 123 +/- 12 to 543 +/- 175 ng/cm, hr; eosinophil cationic protein from 80 +/- 23 to 686 +/- 262 ng/cm, hr). Albumin, as a marker of plasma leakage, was also significantly increased.
These data indicate that mast cells and eosinophils are effector cells not only in patients with allergic disease but also in patients intolerant to foods and lacking circulating antibodies. The underlying mechanisms may be a reaction mediated by locally appearing antibodies or an immunologic activation resembling that found in intestinal disorders such as celiac disease.

0 Bookmarks
 · 
71 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Adverse reactions to food may be produced by several mechanisms and present a great diversity of symptoms, which may be reproducible or not reproducible (occasional adverse food reactions). The reproducible adverse food reactions to minimal or usual amounts of food are classified as hypersensitivity reactions, which may be derived from immune disorders (food allergies) or derived from non-immune conditions (food intolerance). Here, we review the clinical presentations of food allergies according to the underlying mechanisms and causative agents in order to put perspectives over the treatment possibilities.
    Journal of Allergy & Therapy. 03/2013; S3:4.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Gastrointestinal food allergy, a well-recognized clinical entity, has a wide spectrum of clinical features, including cutaneous, respiratory and gastrointestinal symptoms and objective abnormalities. The gastrointestinal alterations in food allergy have been described throughout the gastrointestinal tract. Recurrent abdominal pain (RAP) is a common complaint in school-age children. The findings among children with RAP of an underlying food allergy associated with mucosal pathology of the foregut may support a causal relationship between food allergy and RAP. Further studies are needed to elucidate whether well-documented food allergy (based on double-blind placebo-controlled food challenges) is a major cause of RAP.
    Acta Paediatrica 02/2001; 90(1). · 1.97 Impact Factor
  • [Show abstract] [Hide abstract]
    ABSTRACT: Majority of the patients with perceived food hypersensitivity have irritable bowel syndrome (IBS), and a significant proportion of IBS patients also attribute their gastrointestinal complaints to food items. Different factors such as disturbed intestinal fermentation, enteric dysmotility, post-infectious changes and altered microbial flora in the colon as well as psychological disturbances likely play a role in the pathophysiology and symptoms generation in patients with food hypersensitivity. In addition, a number of studies in these patient groups indicate that local, systemic and mucosal immune systems are activated. The question now is no longer intestinal immune activation, but how the immune system is activated in these patients. In the following review, the potential pathogenetic role of the immune system and evidence of immune activation are reported in patients with perceived food hypersensitivity.
    Digestive Diseases and Sciences 11/2013; · 2.26 Impact Factor