Identification of avian dendritic cells in the spleen using a monoclonal antibody specific for chicken follicular dendritic cells.
ABSTRACT In the chicken, circulating antigens enter the splenic white pulp via the Schweigger-Seidel sheaths (ellipsoids), where they are bound by cells, the ellipsoid associated cells (EAC), which are located on the periphery of the ellipsoid. There is an increasing body of evidence that these antigen-binding cells move through the PALS, to be finally located within the germinal centers, where these antigen-transporting EAC function as follicular dendritic cells (FDC). The aim of the current study was to further study the relationship between the EAC, the FDC, and the antigen-bearing EAC which migrate through the splenic white pulp.
In order to identify the splenic FDC and their presumed migrating EAC precursors in the chicken, we used a monoclonal antibody produced against chicken FDC and an antiserum anti-S-100 protein which identifies chicken dendritic cells in lymphoid organs.
Cells reacting with the 74.3 monoclonal antibody, which identifies FDC, were found within the germinal center, around the penicilliform capillary, in the periellipsoidal white pulp, and in the periarteriolar lymphatic sheaths (PALS). S-100+ cells were found in these same locations.
A comparison between the staining patterns obtained with both antibodies strongly suggested that the intrasplenic distribution of 74.3+ cells was identical with that of FDC, EAC, and antigen-binding EAC migrating in the PALS. Therefore, the 74.3 monoclonal antibody identified not only FDC but also the splenic precursor cells of FDC, in accordance with the hypothesis of the migration of the EAC through the white pulp. S-100+ cells were more numerous than 74.3+ cells, which is in accordance with the fact that S-100 protein antibody stains both FDC and interdigitating dendritic cells (ID). This has allowed us to suggest that 74.3- EAC may represent precursors of ID. The current findings reinforce previous investigations, which provided evidence supporting the migration of EAC through the PALS and further supported the hypothesis which considers EAC precursors of FDC.
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ABSTRACT: The chicken spleen was studied immunohistochemically with anti-S-100 protein polyclonal antibody. S-100-positive cells accumulated around the penicilliform capillaries during the first 3 weeks of life. After 2 weeks posthatch the S-100-positive cells appeared in the red pulp, periarterial lymphatic sheath, and subsequently in the germinal center. Their ontogenetic development and intrasplenic distribution strongly suggested that the S-100-positive cells were identical with ellipsoid-associated cells. The S-100-negative cells of the periellipsoidal white pulp gradually transformed to S-100-positive, functionally active cells on the surface of the ellipsoid. The immunohistological findings support the hypothesis that the interdigitating dendritic cells and follicular dendritic cells were not of monocytic origin but belong to a splenic resident, endocytic cell line located on the surface of the ellipsoid.Developmental & Comparative Immunology 01/1993; 17(1):77-83. · 3.24 Impact Factor
- Nature: New biology 05/1973; 242(121):241-4.
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ABSTRACT: In the present study S-100 protein containing cells in the caecal tonsil were investigated, both at light microscopic and at electron microscopic levels, after oral coccidia inoculation (Eimeria tenella). The birds were infected with a single (Day 0) or two (Days 0 and 21) infective doses of 500 oocysts. Immunoelectron reactivity for S-100 protein was demonstrated in infected chickens, but not in controls. It was found in follicular dendritic cells and interdigitating dendritic cells which were present in the germinal center and diffuse lymphoid tissue respectively, both of them being located in the deep lymphoid tissue near the muscular layer and around the deep glands. Outside the lymphoid tissue, immunoreactivity for S-100 protein was found in cells lying between the epithelial cells of the deep crypt epithelium. Positivity for S-100 protein was observed at 3, 12, 18, 24 and 48 h after the first inoculation as well as on Days 3, 5, 6, 7, 8, 25 and 30 of the experiment. Positive reaction for S-100 protein was detected both while schizont (the more immunogenic stage) development occurs and the number of sporozoites in the caeca lumen was higher, as well as when the production of oocysts reached a maximum. Complementary studies demonstrated that S-100 positive dendritic cells gave a negative reaction for esterase activity, whereas a subset of S-100 negative intraepithelial lymphocytes located between epithelial cells lining the deep glands exhibited esterase activity. These esterase positive cells are hypothesized to be involved in the regulation of local defences.Veterinary Immunology and Immunopathology 10/1993; 38(1-2):123-37. · 1.88 Impact Factor