Article

Mapping of 228 ESTs and 26 Genes into an Integrated Physical and Genetic Map of Human Chromosome 17

Department of Cancer Biology, Lerner Research Institute, Cleveland Clinic Foundation, Ohio 44195, USA.
Genomics (Impact Factor: 2.79). 11/1997; 45(1):140-6. DOI: 10.1006/geno.1997.4906
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ABSTRACT We have integrated genetic and physical mapping data for chromosome 17 subdivided into 26 bins, by using a panel of chromosome 17 deletion somatic cell hybrids. One hundred four short tandem repeat and STS markers have been localized into these bins and have enabled the ordering of 288 ESTs and 26 genes, including 142 ESTs that had not been previously sublocalized on chromosome 17. The mapping information of several genetic maps, as well as information obtained by radiation hybrid and STS content mapping of YACs, has been integrated using this hybrid panel. Although existing mapping information for chromosome 17 was generally consistent for many ESTs previously mapped, the map presented here further refines the location of ESTs, as well as demonstrating a number of discrepancies found in the 17q24-q25 region. We attribute these discrepancies to the fact that the current radiation hybrid panels were selected for retention of the thymidine kinase gene at 17q25, as well as to a low concentration of YAC contigs in this region. These data illustrate the benefit of combining multiple mapping techniques to obtain the greatest accuracy. The integration of maps developed by different methods will generate the most accurate genome maps, which may then be used for the generation of large insert clone contigs for chromosome sequencing. Additionally, accurate transcript maps generated by ESTs will greatly speed the isolation of genes linked to disease loci.

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    • "The vast majority of the clones were selected from human chromosome 17 database resources, such as the Sanger Institute and the Weizmann Institute, as well as genome resources, such as the National Center for Biotechnology Information (NCBI), for their content of STSs, ESTs, or genes described previously in the 17p13.3 region (Stack et al. 1995; Plummer et al. 1997; Hoff et al. 2000; McHale et al. 2000). All genomic clones were purchased from Research Genetics. "
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