Effects of H2 receptor blocking agents on bacterial translocation in burn injury.
ABSTRACT We experimentally studied the effects of H2 receptor blockers (ranitidine) on bacterial translocation (BT) in 42 male albino rats. Sham group (Group I, n = 12 rats) were exposed to 21 degrees C water while Burn group (Group II, n = 15 rats) and Ranitidine group (Group III, n = 15 rats) were exposed to 95 degrees C hot water for 10 seconds to produce a full thickness burn in 30% of total body surface area. 300 mg/kg ranitidine was administered to Group III starting immediately after the burn injury. Rats were sacrificed on the fifth postburn day. Sham group gained weight while groups II and III had significant weight loss. Gastric pH increased with the administration of ranitidine. Both gram negative and total number of bacteria were found to be reduced in cecal stool cultures in ranitidine group. Significant increase in BT was observed in Group III, and translocating bacteria were found to be different in burn and ranitidine groups with a final conclusion that administration of ranitidine changes intestinal ecological equilibrium and promotes BT.
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ABSTRACT: We experimentally studied the effects of antithrombin III (AT III) on bacterial translocation (BT) and intestinal morphology in the early period of burn injury. For this aim, 30 male albino rats were used. A sham burn group (group 1, no. 10) was exposed to 21 °C water. A burn group (group 2, no. 10) and a burn + AT III group (group 3, no. 10) were exposed to 95 °C water for 10 sec, producing full-thickness burn in 30% of the total body surface area. In group 3 the rats received 250 U/kg AT III via the right jugular vein, 15 min before burn injury. One ml 0.9% NaCl was given as a placebo in group 1 and in two rats by the same route. All group 3 rats were sacrificed on day 2 post-burn using an overdose anaesthetic. Cultures of the mesenteric lymph nodes, liver, spleen, blood, and caecal contents were performed. Histopathological examinations, including polymorph nuclear leukocyte (PNL) infiltration and villus morphologies, were qualitatively evaluated on the resected distal ileal segment. The incidence of BT was 1/10 (10%) in group 1, 7/10 (70%) in group 2, and 1/10 (10%) in group 3. A significant increase in BT incidence was observed in group 2 compared with groups 1 and 3 (p = 0.02), while a significant decrease in BT incidence was found in group 3 rats with AT III treatment. Although the PNL infiltration rate was reduced by AT III treatment, a significant decrease was not found compared with group 2 (50% and 90%, respectively). On the other hand, the villus degeneration rate was significantly reduced by AT III treatment compared with group 2 (30% and 90%, respectively). These results suggest that the incidence of BT was enhanced by the burn injury. AT III decreased the incidence of BT in the early period of burn injury. We conclude that AT III can be effectively used to protect from intestinal mucosal injury and to prevent bacterial translocation, especially in early post-burn period.Annals of burns and fire disasters. 12/2006; 19(4):196-200.
- Critical Care Medicine 09/2002; 30(8):1912-3. · 6.12 Impact Factor
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ABSTRACT: To investigate the regulatory effect of histamine on the barrier function of intestinal mucosal. The monolayer Caco-2 cell system in vitro and the model of hemorrhage infection in rats in vivo were established as experimental models. The amount of bacterial translocation was taken as an index of the effect of histamine and its receptor antagon, cimetidine on the intestinal mucosal barrier function. (1) The in vitro experiment showed that after treatment with histamine, the CFU of Escherichia coli 075 invading into Caco-2 cells were much lower than that in the control group (P < 0.05). (2) The animal experiment showed that in the histamine group (hemorrhage infection rats treated with histamine), the average numbers of bacteria in the liver and lymph nodes were much lower than that in control group (P < 0.05). The mean bacterial number in the cimetidine group (hemorrhage infection rats treated both with histamine and cimetidine) was more than that in the histamine group, but without statistical signification (P > 0.05). But the rate of translocation to the liver between histamine group (37.5%) and cimetidine group (100%) was statistically different (P < 0.05) Small concentration of histamine can inhibit bacteria from entering epithelial cells and inhibit intestinal bacterial translocation.Journal of Gastrointestinal Surgery 07/2010; 14(7):1180-5. · 2.36 Impact Factor