Article

Short telomeres on human chromosome 17p. Nat Genet 18: 76-80

Terry Fox Laboratory for Hematology/Oncology, British Columbia Cancer Research Centre, Vancouver, Canada.
Nature Genetics (Impact Factor: 29.65). 02/1998; 18(1):76-80. DOI: 10.1038/ng0198-018
Source: PubMed

ABSTRACT Human chromosomes terminate in a series of T2AG3 repeats, which, together with associated proteins, are essential for chromosome stability. In somatic cells, these sequences are known to be gradually lost through successive cells divisions; however, information about changes on specific chromosomes is not available. Individual telomeres could mediate important biological effects as was shown in yeast, in which loss of a single telomere results in cell-cycle arrest and chromosome loss. We now demonstrate by quantitative fluorescence in situ hybridization (Q-FISH; ref. 7) that the number of T2AG3 repeats on specific chromosome arms is very similar in different tissues from the same donor and varies only to some extent between donors. In all sixteen individuals studied, telomeres on chromosome 17p were shorter than the median telomere length--a finding confirmed by analysis of terminal restriction fragments from sorted chromosomes. These observations provide evidence of chromosome-specific factors regulating the number of T2AG3 repeats in individual telomeres and raise the possibility that the relatively short telomeres on chromosome 17p contribute to the frequent loss of 17p alleles in human cancers.

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    • "Quantitation of telomere length using a telomeric peptide nucleic acid (PNA) probe was done for the first time in 1996 by Lansdorp et al. [32]. Chromosome-specific features were proposed to control telomere length because particular telomere lengths were associated with specific chromosomes [33]. Telomeres on specific chromosome arms were also found to have interallelic differences, through the use of STELA, a polymerase chain reaction–based technique [34] [35]. "
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    Neoplasia (New York, N.Y.) 04/2012; 14(4):344-51. DOI:10.1593/neo.12446 · 5.40 Impact Factor
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    • "The shortest telomere 17p in the healthy population was previously suggested to be associated with human cancers, because the p53 gene and other potential tumor-suppressor genes are located on 17p [20] [38]. Figure 4. Dot plots for z value analysis of " acquired " long RTLs. "
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    ABSTRACT: Previous studies demonstrated that critically shortened telomere lengths correlate with the chromosome instability in carcinogenesis. However, little has been noticed regarding the correlation of long telomeres at specific chromosomes with malignant disorders. We studied relative telomere lengths (RTLs) for individual chromosomes using the quantitative fluorescence in situ hybridization technique in a cohort of 32 patients with chronic myeloid leukemia (CML) and 32 normal samples. We found that telomeres at some specific chromosome arms remain well maintained or even lengthened in a high frequency (27/32) of leukemia cases. In particular, 10 chromosome arms, 4q, 5p, 7q, 11p, 13p, 13q, 14p, 15p, 18p, and Xp, with long telomeres were consistently identified in different samples, and six of them (4q, 5p, 13p, 13q, 14p, and Xp) with relatively long telomeres were also observed in normal samples, but they appeared in lower occurrence rate and shorter RTL than in CML samples. Our results strongly indicate the presence of a special leukemia cell population, or a clone, originated from a common progenitor that is characterized with chromosome arm-specific long telomeres. We suggest that relatively long telomeres located at key chromosomes could be preferentially maintained or further elongated during the early stage of malignant transformation.
    Neoplasia (New York, N.Y.) 06/2011; 13(6):550-60. DOI:10.1593/neo.11358 · 5.40 Impact Factor
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    • "These two techniques provide a global picture of telomere lengths at the genomic and cellular levels by assessing their average length. The advent of telomere length measurement by quantitative FISH (Q-FISH) [16] has allowed a better understanding of telomere length at the chromosomal level. Besides the t(9;22)(q34;q11.2) translocation, other chromosomal aberrations have been reported during the progression of CML [17], and the critical shortening of telomeres may be related to these chromosomal changes. "
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    Neoplasia (New York, N.Y.) 11/2009; 11(11):1146-54. DOI:10.1593/neo.09836 · 5.40 Impact Factor
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