Article

Changes in force and cytosolic Ca2+ concentration after length changes in isolated rat ventricular trabeculae.

Department of Pharmacology, United Medical and Dental Schools, St Thomas's Hospital, London, UK.
The Journal of Physiology (impact factor: 4.72). 02/1998; 506 ( Pt 2):431-44. pp.431-44
Source: PubMed

ABSTRACT 1. Changes in cytosolic [Ca2+] ([Ca2+]i) were measured in isolated rat trabeculae that had been micro-injected with fura-2 salt, in order to investigate the mechanism by which twitch force changes following an alteration of muscle length. 2. A step increase in length of the muscle produced a rapid potentiation of twitch force but not of the Ca2+ transient. The rapid rise of force was unaffected by inhibiting the sarcoplasmic reticulum (SR) with ryanodine and cyclopiazonic acid. 3. The force-[Ca2+]i relationship of the myofibrils in situ, determined from twitches and tetanic contractions in SR-inhibited muscles, showed that the rapid rise of force was due primarily to an increase in myofibrillar Ca2+ sensitivity, with a contribution from an increase in the maximum force production of the myofibrils. 4. After stretch of the muscle there was a further, slow increase of twitch force which was due entirely to a slow increase of the Ca2+ transient, since there was no change in the myofibrillar force-[Ca2+]i relationship. SR inhibition slowed down, but did not alter the magnitude of, the slow force response. 5. During the slow rise of force there was no slow increase of diastolic [Ca2+]i, whether or not the SR was inhibited. The same was true in unstimulated muscles. 6. We conclude that the rapid increase in twitch force after muscle stretch is due to the length-dependent properties of the myofibrils. The slow force increase is not explained by length dependence of the myofibrils or the SR, or by a rise in diastolic [Ca2+]i. Evidence from tetani suggests the slow force responses result from increased Ca2+ loading of the cell during the action potential.

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Keywords

Ca2+ loading
 
diastolic [Ca2+]i
 
force-[Ca2+]i relationship
 
length dependence
 
length-dependent properties
 
maximum force production
 
muscle length
 
myofibrillar Ca2+ sensitivity
 
myofibrillar force-[Ca2+]i relationship
 
rapid potentiation
 
rat trabeculae
 
slow force increase
 
slow force response
 
slow force responses result
 
SR inhibition
 
SR-inhibited muscles
 
twitch force
 
twitch force changes
 
twitches
 
unstimulated muscles