Strongyloides stercoralis and other enteroparasites in children at Uberlândia city, state of Minas Gerais, Brazil.

Departmento de Patologia, Universidade Federal de Uberlândia, MG, Brasil.
Memórias do Instituto Oswaldo Cruz (Impact Factor: 1.36). 01/1998; 93(2):161-4. DOI:10.1590/S0074-02761998000200004
Source: PubMed

ABSTRACT To evaluate the rate of infection by Strongyloides stercoralis and other enteroparasites a survey was conducted in the city of Uberlândia, State of Minas Gerais, Brazil. A total of 900 stool samples from 300 children aging from four months to seven years, randomly selected in ten nursery schools from September 1994 to December 1995, were examined, both by the Baermann-Moraes and Lutz methods. Thirty nine children (13%) were found to be infected by S. stercoralis, 64.1% were boys and 35.9% were girls. Taking all the enteroparasites as a whole the results of the survey pointed out that 265 (88.4%0 of the 300 children were infected by the following: Giardia lamblia, 78.3%; Ascaris lumbricoides, 15.3%; S. stercoralis, 13%; Hymenolepis nana, 6.7%; hookworms, 6%; Enterobius vermicularis, 4%; Hymenolepis diminuta, 4% and Trichuris trichiura, 0.7%. From 265 infected children 64.5% were mono-infected, 27.2% were infected by two parasites and 8.3% had a poly-specific parasite burden. It was concluded that strongyloidiasis is hyperendemic in this area.

0 0
  • Source
    [show abstract] [hide abstract]
    ABSTRACT: Taenia saginata metacestode antigens have been constituted a useful alternative antigen for neurocysticercosis (NC) serodiagnosis, particularly due to an increasing difficulty to obtain Taenia solium homologous antigen. Cross-reactivity with Echinococcus granulosus infection occurs in homologous and heterologous antigens and could be avoided by using different purified methods. The present study evaluated antigen fractions obtained from saline extracts of T. saginata metacestodes purified by affinity chromatography with jacalin or concanavalin A (ConA) lectins to detect IgG antibodies by enzyme-linked immunosorbent assay (ELISA) and immunoblot analysis to diagnose human NC. Serum samples were collected from 142 individuals: 40 of them were diagnosed with NC, 62 presented Taenia sp. and other parasites, and 40 were apparently healthy individuals. The jacalin- and ConA-unbound fractions demonstrated sensitivity and specificity higher than those of bound fractions. Among unbound fractions, ConA demonstrated statistically higher sensitivity and specificity by ELISA (90% and 93.1%, respectively). By immunoblot assay, the 64- to 68-kDa component from the ConA-unbound fraction showed 100% sensitivity and specificity, making this component suitable for use as a specific antigen for diagnosis of NC. To our knowledge, this is the first report showing the relevance of using the unbound ConA fraction of T. saginata metacestodes to diagnose NC. In conclusion, the results obtained herein clearly demonstrate that antigenic fractions without affinity to ConA, obtained from T. saginata metacestodes, are an important source of specific peptides and are efficient in the diagnosis of NC when tested by immunoblot assay.
    Clinical and vaccine Immunology: CVI 04/2010; 17(4):638-44. · 2.60 Impact Factor
  • [show abstract] [hide abstract]
    ABSTRACT: Strongyloidiasis, caused by the nematode Strongyloides stercoralis, is one of the major worldwide parasitic infections in humans. Breastfeeding may offer a potential protection against this infection. Feces, serum and milk samples were obtained from 90 lactating women from Clinical Hospital of Universidade Federal de Uberlândia, Brazil. The fecal samples were collected for parasitological diagnosis and the serum and milk samples were examined for specific S. stercoralis IgA and IgG antibodies using the indirect fluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA). Fecal examination showed that the rate of prevalence of S. stercoralis infection in the lactating women was 4.4%. IFAT manifested a 16.7% positivity rate for specific IgA antibody in serum and a 28.9% rate in milk samples; specific IgG was 41.1% in serum and 25.5% in milk samples. According to ELISA the positivity rate for specific IgA antibody was 21.1% in serum and 42.2% in milk samples; specific IgG was 40% in serum and 18.9% in milk samples. In serum samples, these immunological tests showed a concurrence of 91.1% and 94.4%, respectively, in detecting specific IgA and IgG antibodies. In milk samples, they showed a concurrence of 70% and 78.9%, respectively, in detecting specific IgA and IgG antibodies. There was a statistically significant difference between concordant and discordant results of immunological tests (P<0.0001). IFAT and ELISA highly concurred in their detection of specific S. stercoralis IgA and IgG antibodies in serum and in milk samples reconfirming prior studies that the serological method is a complement to the direct diagnosis of the parasite, and suggesting that immunological methods using milk samples can also be helpful. Furthermore, in endemic areas, infants may acquire antibodies to S. stercoralis from breast milk, possibly, contributing to the enhancement of specific mucosal immunity against this parasite.
    Acta tropica 11/2008; 109(2):103-7. · 2.79 Impact Factor
  • Source
    [show abstract] [hide abstract]
    ABSTRACT: The aim of this study was to verify the occurrence of Strongyloides stercoralis and other enteroparasites in the rural area of Uberlândia, Minas Gerais, Brazil, between September 1996 and May 1997, using the Baermann -Moraes and Lutz methods. Out of 180 individuals studied, 92 (51.1%) were infected. Twelve people (6.7%) were infected by S. stercoralis, being five in children with ages between zero and 12 years old and 7 cases older than 12 years old. Other enteroparasites diagnosed were Entamoeba coli (27.2%), Giardia lamblia (10.6%), Hookworm (7.2%), Endolimax nana (3.9%), Ascaris lumbricoides (3.3%), Entamoeba hartmanni (3.3%), Enterobius vermicularis (2.2%), Hymenolepis nana (1.1%), Iodamoeba butschlii (1.1%), Entamoeba histolytica/ dispar (0.6%), and Schistosoma mansoni (0.6%). Of the 92 positive cases there were 71% with mono-parasitism, 25% with bi-parasitism, and 4% with poly-parasitism. It was concluded that strongyloidiasis is hyperendemic in the rural area of Uberlândia. We highlighted the importance of the multiple parasitological methods performed in three different occasions with fecal samples of each individual, which enhanced the possibilities for detection of an elevated enteroparasite rate (51.1%). We also point out to a serious public health problem in this rural area.
    01/2010; 39:115-122.