Article

Epitopes on beta2-GPI recognized by anticardiolipin antibodies.

Department of Medicine II, Hokkaido University School of Medicine, Sapporo, Japan.
Lupus (impact factor: 2.34). 02/1998; 7 Suppl 2:S14-7. pp.S14-7
Source: PubMed

ABSTRACT Anticardiolipin antibodies (aCL) found in sera from patients with antiphospholipid syndrome recognize a cryptic epitope that appears on the beta2-glycoprotein I (beta2-GPI) molecule when beta2-GPI interacts with a lipid membrane composed of negatively charged phospholipid or when beta2-GPI is adsorbed on a polyoxygenated polystyrene plate. A homology based model of beta2-GPI was constructed based on the NMR coordinates of sushi domains of human factor H. The conformation was like a cylinder consisting of five domains, its IV and V domains being glued by electrostatic interaction. We used phage-displayed random peptide libraries to search the epitopes of human aCL. Structures similar to consensus sequences selected by a biopanning method was found on domain IV of beta2-GPI.

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Keywords

aCL
 
Anticardiolipin antibodies
 
antiphospholipid syndrome
 
beta2-GPI
 
beta2-GPI interacts
 
biopanning method
 
consensus sequences
 
domain IV
 
electrostatic interaction
 
homology
 
human aCL
 
human factor H
 
phage-displayed random peptide libraries
 
polyoxygenated polystyrene plate
 
sera
 
sushi domains