Article

Gene expression of growth-associated proteins, GAP-43 and SCG10, in the hippocampal formation of the macaque monkey: nonradioactive in situ hybridization study.

Information Science Division, Electrotechnical Laboratory, Umezono, Tsukuba, Ibaraki, Japan.
Hippocampus (impact factor: 5.18). 02/1998; 8(5):533-47. DOI:10.1002/(SICI)1098-1063(1998)8:5<533::AID-HIPO11>3.0.CO;2-# pp.533-47
Source: PubMed

ABSTRACT We performed nonradioactive in situ hybridization histochemistry in the monkey hippocampal formation that includes the hippocampus, the subicular complex, and the entorhinal cortex to detect the expression of mRNA for two growth-associated proteins: GAP-43 and SCG10. Overall, the distribution patterns overlapped but were partially distinct. In the hippocampus, the intense hybridization signals for both GAP-43 and SCG10 mRNAs were observed in the pyramidal cell layer of Ammon's horn, especially in CA3 subfields. The intense hybridization signals were also observed in the stratum oriens of Ammon's horn and the polymorphic layer of the dentate gyrus. In the granule cell layer of the dentate gyrus, many GAP-43 mRNA-positive cells were observed, whereas a few positive cells with weak signals were observed for SCG10 mRNA. Throughout the subicular complex, the hybridization signals for both mRNAs were weak. In the entorhinal cortex, both mRNAs were abundant in the caudal field. These subregion-specific expression of the growth-associated proteins may reflect the functional specialization regarding plasticity in each region of the monkey hippocampal formation.

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Keywords

Ammon's horn
 
CA3 subfields
 
caudal field
 
dentate gyrus
 
distinct
 
distribution patterns overlapped
 
entorhinal cortex
 
GAP-43 mRNA-positive cells
 
granule cell layer
 
growth-associated proteins
 
hybridization signals
 
intense hybridization signals
 
monkey hippocampal formation
 
polymorphic layer
 
positive cells
 
pyramidal cell layer
 
situ hybridization histochemistry
 
stratum oriens
 
subicular complex
 
subregion-specific expression