Article

Recombinant human monoclonal antibodies to Ebola virus.

Department of Immunology, The Scripps Research Institute, La Jolla, California 92037, USA.
The Journal of Infectious Diseases (impact factor: 6.41). 03/1999; 179 Suppl 1:S235-9. DOI:10.1086/514280 pp.S235-9
Source: PubMed

ABSTRACT Human Fab (IgG1kappa) phage display libraries were constructed from bone marrow RNA from 2 donors who recovered from infection with Ebola (EBO) virus during the 1995 outbreak in Kikwit, Democratic Republic of the Congo. The libraries were initially panned against a radiation-inactivated EBO virus-infected Vero cell lysate, but only weak binders were identified. In contrast, panning against secreted EBO glycoprotein (SGP) resulted in Fabs showing very strong reactivity with SGP in ELISA. These Fabs also reacted with a virion membrane preparation. The Fabs were strongly positive in IFAs with cells infected with EBO (subtype Zaire) virus but negative with uninfected cells, with a characteristic punctate staining pattern in the cytoplasm. The Fabs showed weak or no reactivity with the virus cell lysate although donor serum did react. The Fabs are now being characterized in structural and functional terms. Major interest will focus on the ability of antibodies to neutralize EBO virus and, later, to protect animals against infection.

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Keywords

2 donors
 
antibodies
 
bone marrow RNA
 
characteristic punctate staining pattern
 
Democratic Republic
 
donor serum
 
Fabs
 
functional terms
 
Human Fab
 
IFAs
 
Kikwit
 
libraries
 
Major interest
 
panning
 
SGP
 
subtype Zaire
 
uninfected cells
 
virion membrane preparation
 
virus cell lysate
 
weak binders