Strategy for membrane protein crystallization exemplified with OmpA and OmpX.

Institut für Organische Chemie und Biochemie, Albert-Ludwigs-Universität, Freiburg im Breisgau, Germany.
Proteins Structure Function and Bioinformatics (Impact Factor: 3.34). 03/1999; 34(2):167-72.
Source: PubMed

ABSTRACT The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable for a broader range of membrane proteins.

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