Article

Glutathione-independent prostaglandin D2 synthase in ram and stallion epididymal fluids: origin and regulation.

Station de Physiologie de la Reproduction des Mammifères Domestiques, URA INRA-CNRS 1291, 37380 Nouzilly, France.
Biology of Reproduction (impact factor: 4.01). 04/1999; 60(3):558-66. pp.558-66
Source: PubMed

ABSTRACT Microsequencing after two-dimensional electrophoresis revealed a major protein, glutathione-independent prostaglandin D2 synthase (PGDS) in the anterior epididymal region fluid of the ram and stallion. In this epididymal region, PGDS was a polymorphic compound with a molecular mass around 30 kDa and a range of pI from 4 to 7. PGDS represented 15% and 8% of the total luminal proteins present in this region in the ram and stallion, respectively. The secretion of the protein as judged by in vitro biosynthesis, and the presence of its mRNA as studied by Northern blot analysis, were limited to the proximal caput epididymidis. Using a specific polyclonal antibody raised against a synthetic peptide, PGDS was found throughout the epididymis, decreasing in concentration toward the cauda region. PGDS was also detected in the testicular fluid and seminal plasma by Western blotting. Castration and efferent duct ligation in the ram led to a decrease in PGDS mRNA and secretion. PGDS mRNA was not detected in the stallion 1 mo after castration, and it was restored by testosterone supplementation. This study showed that PGDS is present in the environment of spermatozoa throughout the male genital tract. Its function in the maturation and/or protection of spermatozoa is unknown.

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Keywords

anterior epididymal region fluid
 
cauda region
 
epididymal region
 
epididymis
 
glutathione-independent prostaglandin D2 synthase
 
major protein
 
male genital tract
 
molecular mass
 
Northern blot analysis
 
PGDS mRNA
 
proximal caput epididymidis
 
seminal plasma
 
specific polyclonal antibody
 
stallion 1 mo
 
testicular fluid
 
testosterone supplementation
 
total luminal proteins present
 
two-dimensional electrophoresis
 
vitro biosynthesis
 
Western blotting
 

S Fouchécourt