Secretion of Salmonella-specific antibodies in the oviducts of hens experimentally infected with Salmonella enteritidis.
ABSTRACT The production and secretion of Salmonella enteritidis whole cell antigen-specific antibodies in the oviducts and in the serum of laying hens experimentally infected with Salmonella enteritidis, was analyzed by ELISA. The dynamics of the antibody levels in the oviducts were identical to that in the serum. Subclasses of antibodies (IgA, IgG, and IgM) in the infected hens were found to increase significantly (p < 0.01) compared to those in the control uninfected hens throughout the experiment. IgG and IgM levels in both oviducts and in sera reached to a peak by 14 days post-inoculation, and remained elevated throughout. The secretion of IgA seemed to be transient since the IgA levels increased to a peak 7 days after both primary and secondary inoculations, and declined rapidly. The elevated levels of antibodies were followed by partial clearance of Salmonella organisms from the oviducts. The present results indicate a significant local immune reaction against the Salmonella infection and suggest an association of the local antibodies with the clearance of Salmonella from the oviducts at least partially.
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ABSTRACT: Current knowledge of the immunoglobulin classes identified in some avian species is reviewed. The distribution and fate of passively acquired immunoglobulins or specific antibodies in compartments of the egg and of the developing embryo and in the newly hatched chick are described, together with the ontogeny of active Ig biosynthesis.Developmental & Comparative Immunology 02/1981; 5(1):15-20. · 3.24 Impact Factor
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ABSTRACT: Lymphocytes expressing CD3, CD4, CD8, pan lymphocyte, IgA, IgG and IgM cell surface antigens were assessed by in the spleen and thymus of chickens following infection with Salmonella enteritidis using flow cytometric analysis. At 6 days post primary infection and 2 days post secondary infection with S. enteritidis, the percentages of IgA+ and IgM+ lymphocytes in the spleen were significantly increased (P < 0.05). At 2 days post secondary infection with S. enteritidis, the percentage of CD4+ T lymphocyte in the spleen and CD8+ T lymphocyte percentage in the thymus were significantly increased (P < 0.05). These results indicate that S. enteritidis infection induces changes in the spleen and thymus that reflect the dynamics of the host protective immune response.Veterinary Immunology and Immunopathology 11/1997; 59(3-4):359-67. · 1.88 Impact Factor
Article: Salmonella enteritidis in Canada.[show abstract] [hide abstract]
ABSTRACT: During the last few years the prevalence of Salmonella enteritidis in people in Canada has increased slightly from 9 to 12% of all Salmonella isolates. Nation-wide surveys showed a low prevalence of S. enteritidis in poultry flocks: S. enteritidis was isolated from environmental samples of 2.7% of layer flocks and of 3% of broiler flocks. The overall prevalence of S. enteritidis-contaminated eggs from two flocks with infected hens was less than 0.06%. With the exception of S. enteritidis phage type (PT) 4, which has not been isolated from poultry in Canada, the phage types that occur most commonly in poultry (PT 8, 13 and 13a) are also the most prevalent in people. Phage typing was the most effective method for subdividing S. enteritidis strains. Eighty-three percent of the S. enteritidis isolates from poultry and its environment were sensitive to antimicrobial agents. The reasons why strains of the same phage type differ in virulence need to be elucidated.International Journal of Food Microbiology 02/1994; 21(1-2):1-5. · 3.43 Impact Factor