Effect of different anticoagulant, underfilling of blood sample and storage stability on selected hemogram.
ABSTRACT We collected blood samples from 94 adult non-hematological outpatients and inpatients for complete blood count (CBC) without any flagging at Kaohsiung Medical College Hospital in order to investigate the effect of (1) different anticoagulants with Na2 EDTA vs K3 EDTA (2) the underfilling of blood collection volume (2 ml, 3. 5 ml vs standard 5 ml) (3) the difference in storage stability between 1 hour, 4 hours, 8 hours and 12 hours after venesection at room temperature on some selected hemogram parameters (WBC, RBC, hemoglobin, hematocrit, MCV, MCH, MCHC, platelet, percentage of neutrophil and lymphocyte). The automated hematology analyzer we used was SYSMEX NE-8000, (TOA, Japan). All the EDTA collection vacutainer tubes were supplied by Becton-Dickinson (New Jersey, U. S. A.) with the same lot number. Paired t- test was used for statistics. We found that values of hemoglobin, hematocrit, MCV and lymphocyte percentage collected in Na2 EDTA tubes were significantly higher than those collected in K3 EDTA (P < 0.05 for hemoglobin and lymphocyte percentage, and P all < 0.01 for others), while values of MCHC collected in Na2 EDTA were significantly lower than those collected in K3 EDTA (P < 0.05). For underfilling of blood sample, values of hematocrit and MCV with 2 ml blood volume were significantly lower than those with 5 ml blood volume (both P < 0.01), while values of MCHC with 2 ml blood volume were significantly higher than those with 5 ml blood volume (P < 0.01). When the collection blood volume was increased to 3.5 ml, there were no significant difference between values for 3.5 ml and 5ml blood volume (P all > 0.05). In the storage stability study, there was a significant sequential increase of hematocrit and MCV between 1 hour, 8 hours and 12 hours (P < 0.05 and < 0.01, respectively, for 8 hours, P all < 0.01 for 12 hours). There was also a significant sequential decrease of neutrophil percentage between 1 hour and 4, 8, 12 hours' storage at room temperature (P all < 0.01).
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ABSTRACT: The complete blood count is one of the most common routine tests. This study aimed to evaluate possible effects of the antioxidant taurine on the complete blood count of whole blood stored at room temperature and at 4ºC over seven days. Venous blood samples of 25 healthy males were distributed into two sets of tubes with each set of four tubes containing 50 µL of solutions with zero, 2.5 g/L, 5 g/L, 10 g/L taurine. The tubes were kept at room temperature or at 4ºC. Complete blood counts were performed on seven successive days. The mean percentage changes [Δ = (mean value - mean baseline value) / mean baseline value x 100] were calculated and compared. Complete blood count parameters exhibited different patterns of behavior which were affected by the storage temperature, time and taurine concentration. Taurine at room temperature significantly enhanced the stability of: the platelet count over seven days (Δ7 at 2.5, 5 and 10 g/L taurine were 5.45, 6.11, and 5.80 x 10(9) cells/L, respectively); the red blood cell count over five days (Δ5 at 2.5, 5 and 10 g/L taurine were 1.59, 2.79, and 1.98 x 10(12) cells/L, respectively); mean corpuscular hemoglobin over five days (Δ5 at 2.5, 5 and 10 g/L taurine were -0.91,-1.52 and -0.84 fl respectively); and red cell distribution width over two days (Δ2 at 2.5, 5 and 10 g/L taurine were 0.90%, 1.30% and -0.1%, respectively). No additional stabilizing effects of taurine were reported for the mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, hematocrit and hemoglobin, while it negatively affected the white blood cell stability. Complete blood count parameters exhibited variable stability patterns in respect to temperature, time and taurine concentration.Revista Brasileira de Hematologia e Hemoterapia 01/2013; 35(1):44-51.
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ABSTRACT: Paired blood samples were collected from the ear and radial vein of four captive healthy adult black rhinoceroses (Diceros bicornis). Samples were collected using heparin or ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. Packed cell volume (PCV) and total protein (TP) values were compared between samples drawn from the two venipuncture sites and treated with the two anticoagulants to determine whether statistically significant variation occurred. No significant difference in the grouped values was observed when venipuncture sites (ear and radial vein) were compared using the same anticoagulant (heparin). However, when comparing different anticoagulants (EDTA and heparin) used to collect blood from the radial vein, the grouped-heparinized samples had higher mean PCV and TP values than did the EDTA-treated samples. These differences may be important when performing serial sampling in a sick rhinoceros and suggest that the choice of anticoagulant should be consistent, although selection of venipuncture site may be less important when monitoring selected hematologic values in black rhinoceroses.Journal of Zoo and Wildlife Medicine 04/2003; 34(1):59-64. · 0.43 Impact Factor