Article

Antibodies to yeast Sm motif 1 cross-react with human Sm core polypeptides.

IIBB-CSIC, Barcelona, Spain.
European Journal of Biochemistry (impact factor: 3.58). 05/1999; 261(2):371-8. pp.371-8
Source: PubMed

ABSTRACT Two regions common to all UsnRNP core polypeptides have been described: Sm motif 1 and Sm motif 2. Rabbits were immunized with a 22 amino-acid peptide containing one segment of Sm motif 1 (YRGTLVSTDNYFNLQLNEAEEF, corresponding to residues 11-32) from yeast F protein. After immunization, the rabbit sera contained antibodies that not only reacted specifically with the peptide from yeast F protein but also cross-reacted with Sm polypeptides from mammals; that is, with purified human U1snRNPs. The results suggest that the peptide used and human Sm polypeptides contain a common feature recognized by the polyclonal antibodies. A large collection of human systemic lupus erythematosus sera was assayed using the yeast peptide as an antigen source. Seventy per cent of systemic lupus erythematosus sera contain an antibody specificity that cross-reacts with the yeast peptide.

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Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

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Keywords

22 amino-acid peptide
 
antibodies
 
antibody specificity
 
antigen source
 
common feature
 
cross-reacted
 
human Sm polypeptides
 
human systemic lupus erythematosus sera
 
peptide
 
polyclonal antibodies
 
purified human U1snRNPs
 
regions common
 
Sm motif 1
 
Sm motif 2. Rabbits
 
Sm polypeptides
 
systemic lupus erythematosus sera
 
UsnRNP core polypeptides
 
yeast F protein
 
yeast peptide
 
YRGTLVSTDNYFNLQLNEAEEF