High-performance liquid chromatographic assay of (+/-)-lactic acid and its enantiomers in calf serum.

College of Pharmacy and Nutrition and The Department of Internal Medicine, University of Saskatchewan, Saskatoon, Canada.
Journal of chromatography. B, Biomedical sciences and applications 04/1999; 727(1-2):23-9. DOI: 10.1016/S0378-4347(99)00072-9
Source: PubMed

ABSTRACT Two high-performance liquid chromatographic methods are described for the determination of lactic acid and its enantiomers in calf serum. A 300x8.0 mm I.D. column packed with sulfonated styrene-divinylbenzene copolymer and a 50x4.6 mm ODS column with N,N-dioctyl-L-alanine were used. UV detection was at 205 and 236 nm for the non-chiral and chiral assays, respectively. Both assays demonstrated excellent linear relationships between peak area ratios and serum concentrations over a range of 0.5 to 20 mM, based on 100 microl bovine serum. Recovery was complete. Inter- and within-batch bias and relative standard deviation were <15%.

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