Article

A small nucleolar RNA:ribozyme hybrid cleaves a nucleolar RNA target in vivo with near-perfect efficiency.

Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst, MA 01003, USA.
Proceedings of the National Academy of Sciences (Impact Factor: 9.81). 07/1999; 96(12):6609-14. DOI: 10.1073/pnas.96.12.6609
Source: PubMed

ABSTRACT A hammerhead ribozyme has been localized to the yeast nucleolus by using the U3 small nucleolar RNA as a carrier. The hybrid small nucleolar RNA:ribozyme, designated a "snorbozyme," is metabolically stable and cleaves a target U3 RNA with nearly 100% efficiency in vivo. This is the most efficient in vivo cleavage reported for a trans-acting ribozyme. A key advantage of the model substrate featured is that a stable, trimmed cleavage product accumulates. This property allows accurate kinetic measurements of authentic cleavage in vivo. The system offers new avenues for developing effective ribozymes for research and therapeutic applications.

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Available from: Dmitry Samarsky, May 16, 2014
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