Secretory leukocyte protease inhibitor (SLPI) concentrations in cervical mucus of women with normal menstrual cycle

Department of Obstetrics and Gynecology, Faculty of Medicine, Osaka University, 2-2 Yamada-oka, Suita City, Osaka 565-0871, Japan.
Molecular Human Reproduction (Impact Factor: 3.48). 08/1999; 5(7):656-61. DOI: 10.1093/molehr/5.7.656
Source: PubMed

ABSTRACT Secretory leukocyte protease inhibitor (SLPI) is a potent inhibitor of human leukocyte elastase. SLPI transcripts in the cervical tissue were detected during the menstrual cycle by reverse transcription-polymerase chain reaction (RT-PCR). Western blot analysis revealed that the intensity of SLPI protein in cervical tissue in the ovulatory phase was stronger than in other phases. Immunohistochemistry using an anti-SLPI polyclonal antibody revealed positive staining in the epithelial cells of the endocervix. Western blot analysis also revealed that SLPI protein was present in the cervical mucus. Again the intensity of SLPI protein in the ovulatory phase was stronger than that in the follicular phase. The SLPI concentrations and SLPI/elastase ratios in the cervical mucus of women in the ovulatory phase were significantly higher than in the follicular and luteal phases. The SLPI and elastase concentrations in the cervical mucus were positively correlated. No significant difference was found in the SLPI serum concentrations of women during the menstrual cycle. These results suggest that production of SLPI from cervical epithelial cells during the ovulatory phase may be important for protection from the effects of elastase.

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Available from: Tadashi Kimura, Aug 20, 2015
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    • "We observed that transcytosis of HIV across polarized endometrial and cervical epithelia did not affect viral infectivity (Figure 3), and this was well correlated with the absence or lowlevel expression of HBD2, HBD3, and SLPI in these cells (Figure 4). Expression of HBD2, HBD3, and SLPI in the genital mucosa was not stable and depends on the menstrual cycle, i.e., HBD3 and SLPI express during the secretory phase, and HBD2 expression has been detected only during menstruation (King, Critchley, and Kelly, 2003;King et al., 2003;Moriyama et al., 1999). "
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    • "mRNA highest at menstruation (Fleming et al. 2003) mRNA highest in secretory phase (King et al. 2003b) mRNA highest in proliferative phase (King et al. 2003b) mRNA and protein highest in secretory phase (King et al. 2000) mRNA and protein highest at menstruation (King et al. 2003a) Cervix mRNA (Valore et al. 1998) Not reported Not reported Not reported Protein (Moriyama et al. 1999) Protein (Pfundt et al. 1996) Vagina mRNA (Valore et al. 1998, Narvekar et al. 2007) mRNA (Narvekar, et al. 2007) mRNA (Narvekar et al. 2007) Not reported Protein – cell line (Fichorova & Anderson 1999, Valore et al. 2006) Protein (Pfundt et al. 1996) mRNA (Narvekar, et al. 2007) Pregnant Amnion mRNA and protein (King et al. 2007) mRNA and protein (King et al. 2007, Stock et al. 2007) mRNA and protein (King et al. 2007) Not reported Protein (Zhang et al. 2001) mRNA and protein (King et al. 2007) Chorion mRNA and protein (King et al. 2007) mRNA and protein (King et al. 2007) mRNA and protein (King et al. 2007) Not reported Mainly negative (Denison et al. 1999) mRNA and protein (King et al. 2007) Placenta mRNA and protein (King et al. 2007) mRNA and protein (King et al. 2007) mRNA and protein (King et al. 2007) Not reported Mainly negative (Denison et al. 1999) mRNA and protein (King et al. 2007) Decidua mRNA and protein (King et al. 2007) mRNA and protein (King et al. 2007) mRNA and protein (King et al. 2007) Not reported mRNA and protein (Denison et al. 1999, King et al. 2000) mRNA and protein (King et al. 2007) Cervical mucus plug Protein (Hein et al. 2002) Not reported Not reported Not reported Protein (Helmig et al. 1995, Hein et al. 2002) "
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    • "The cervical mucus is well known as a multifactorially determined filtering system, but not much is known about its antimicrobial activity (Eggert-Kruse et al., 2000). Even though antibacterial activity of cervical mucus had been found previously , none of the antimicrobial peptides, except SLPI (Moriyama et al., 1999) and lysozyme (Eggert-Kruse et al., 2000), had been purified from the cervical mucus. The aims of the present investigation were to identify novel antibacterial molecules and characterize these molecules as potentially important factors in the innate immunity of human reproductive tract. "
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