DIO-1 is a gene involved in onset of apoptosis in vitro, whose misexpression disrupts limb development

Department of Immunology and Oncology, Centro Nacional de Biotecnología, Universidad Autónoma, Campus de Cantoblanco, E-28049 Madrid, Spain.
Proceedings of the National Academy of Sciences (Impact Factor: 9.81). 08/1999; 96(14):7992-7. DOI: 10.1073/pnas.96.14.7992
Source: PubMed

ABSTRACT The DIO-1 (death inducer-obliterator-1) gene, identified by differential display PCR in pre-B WOL-1 cells undergoing apoptosis, encodes a putative transcription factor whose protein has two Zn finger motifs, nuclear localization signals, and transcriptional activation domains, expressed in the limb interdigitating webs during development. When overexpressed, DIO-1 translocates to the nucleus and activates apoptosis in vitro. Nuclear translocation as well as induction of apoptosis are lost after deletion of the nuclear localization sequences. DIO-1 apoptotic induction is prevented by caspase inhibitors and Bcl-2 overexpression. The in vivo role of DIO-1 was studied by misexpressing DIO-1 during chicken limb development. The most frequently observed phenotype was an arrest in limb outgrowth, an effect that correlates with the inhibition of mesodermal and ectodermal genes involved in this process. Our data demonstrate the ability of DIO-1 to trigger apoptotic processes in vitro and suggest a role for this gene in cell death during development.

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Available from: Juan P Albar, Aug 10, 2015
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    • "We show here that a novel component of the SC, deathinducer obliterator 3 (DIDO3), is incorporated depending on synapsis and H3K4Me3 demethylation. The mammalian Dido gene is expressed in all tissues (García-Domingo et al. 1999) and encodes three proteins termed DIDO1, DIDO2, and DIDO3 through alternative splicing (Fütterer et al. 2005). Dido mutant cells lack the messenger RNA for DIDO1 and DIDO2 (Fütterer et al. 2005), indicating that these two isoforms are unnecessary for survival. "
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    • "The sequences are derived from the following sources. MS1, an Arabidopsis PHD-finger protein involved in anther and pollen development (Wilson et al., 2001); HAT3.1, an Arabidopsis homeobox protein (Schindler et al., 1993); DIO-1, death-associated transcription factor (Garcia-Domingo et al., 1999); ASH1, a putative chromosome-remodeling factor (Nakamura et al., 2000); ZMHOX2a, a maize homeobox gene (Klinge et al., 1996); MLL5, human MIXED-LINEAGE LEUKEMIA5 protein (Emerling et al., 2002). Conserved PHD consensus sequences are shown as open boxes. "
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