Article

In vitro isolation of Neospora caninum from a stillborn calf in the UK.

Veterinary Parasitology, Liverpool School of Tropical Medicine/Faculty of Veterinary Science, Pembroke Place, Liverpool, L3 5QA, UK.
Research in Veterinary Science (Impact Factor: 1.51). 09/1999; 67(1):103-5. DOI: 10.1053/rvsc.1998.0272
Source: PubMed

ABSTRACT Neospora caninum was isolated in Vero cell culture from the brain of a stillborn calf. This isolate (designated NC-LivB1) is the first to be obtained from cattle in the United Kingdom and was confirmed as N. caninum by immunofluorescence with specific antibodies and by internal transcribed spacer 1 (ITS1) sequence analysis. Differences were found between NC-LivB1, other bovine isolates and canine isolates of N. caninum and closely related protozoal parasites, using random amplified polymorphic DNA polymerase chain reaction (RAPD - PCR) techniques.

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    ABSTRACT: Seroprevalence and potential risk factors of Seroprevalence and potential risk factors of Neospora caninum Neospora caninum infection in dairy infection in dairy cattle in Croatia cattle in Croatia. Vet. arhiv 80, 163-171, 2010. ABSTRACT Neospora caninum Neospora caninum, a cyst forming coccidian parasite, is an important cause of abortion in cattle worldwide. , a cyst forming coccidian parasite, is an important cause of abortion in cattle worldwide. The present work aims to update knowledge of the presence of The present work aims to update knowledge of the presence of Neospora Neospora in cattle from Croatia. The survey was in cattle from Croatia. The survey was carried out on dairy cows in the Bjelovar-Bilogora County in northwest Croatia (2652 km carried out on dairy cows in the Bjelovar-Bilogora County in northwest Croatia (2652 km 2 2). The cows were). The cows were selected by simple randomization from county dairy producer records. Cows were tested for the presence of selected by simple randomization from county dairy producer records. Cows were tested for the presence of N. N. caninum caninum antibodies with the Neospora-Ab iscom enzyme-linked immunoassay kit (SVANOVIR). antibodies with the Neospora-Ab iscom enzyme-linked immunoassay kit (SVANOVIR). N. caninum N. caninum antibodies were detected in 23 from 395 (5.6%, confi dence interval (CI); CI 95%; 3.5 to 8.1) lactating cows. antibodies were detected in 23 from 395 (5.6%, confi dence interval (CI); CI 95%; 3.5 to 8.1) lactating cows. Epidemiological studies of potential risk factors, such as the presence of a dog, cat, swine, free range poultry Epidemiological studies of potential risk factors, such as the presence of a dog, cat, swine, free range poultry and management practices, such as silage processing and pasture practising showed that none of these factors and management practices, such as silage processing and pasture practising showed that none of these factors was associated with was associated with N. caninum N. caninum antibodies. Seropositivity of cows was associated to abortion (odds ratio (OR); antibodies. Seropositivity of cows was associated to abortion (odds ratio (OR); OR = 8.307, P = 0.002) and to purchasing of replacement animals from other sources (OR = 5.27, P = 0.0002). OR = 8.307, P = 0.002) and to purchasing of replacement animals from other sources (OR = 5.27, P = 0.0002). A strong correlation was also found between antibodies, history of abortion and origin (imported animals) of A strong correlation was also found between antibodies, history of abortion and origin (imported animals) of heifers or cows (OR = 5.52; P = 0.001). heifers or cows (OR = 5.52; P = 0.001).