Observation of Through-Hydrogen-Bond 2hJHC′ in a Perdeuterated Protein

Institute of Structural Biology, Forschungszentrum Jülich, Jülich, 52425, Germany.
Journal of Magnetic Resonance (Impact Factor: 2.51). 11/1999; 140(2):510-2. DOI: 10.1006/jmre.1999.1899
Source: PubMed


It is demonstrated that J connectivity between amide protons and hydrogen-bond-accepting carbonyl carbons can be observed in perdeuterated human ubiquitin. A selective pulse scheme is used to detect these small 2hJHC' interactions in the presence of the much larger through-covalent-bond 2JHC' and 3JHC' couplings. The ratio of the observed through-H-bond correlation intensity and the 2JHC' connectivity observed in a reference spectrum indicates 2hJHC' values of ca. 0.4-0.6 Hz, which are only slightly smaller than the corresponding 3hJNC' values. However, for technical reasons, 2hJHC' couplings are more difficult to measure than 3hJNC'.

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