Orthogonal polarization spectral imaging: a new method for study of the microcirculation.

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Nature Medicine (Impact Factor: 28.05). 11/1999; 5(10):1209-12. DOI: 10.1038/13529
Source: PubMed
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    ABSTRACT: Nailfold capillaroscopy is a non-invasive and safe technique for the analysis of microangiopathologies. Imaging quality of widely used simple videomicroscopes is poor. The use of green illumination instead of the commonly used white light may improve contrast. The aim of the study was to compare the effect of green illumination with white illumination, regarding capillary density, the number of microangiopathologies, and sensitivity and specificity for systemic sclerosis. Five rheumatologists have evaluated 80 images; 40 images acquired with green light, and 40 images acquired with white light. A larger number of microangiopathologies were found in images acquired with green light than in images acquired with white light. This results in slightly higher sensitivity with green light in comparison with white light, without reducing the specificity. These findings suggest that green instead of white illumination may facilitate evaluation of capillaroscopic images obtained with a low-cost digital videomicroscope.
    Rheumatology International 12/2014; DOI:10.1007/s00296-014-3193-x · 1.63 Impact Factor
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    ABSTRACT: Microcirculation plays a crucial role in physiological processes of tissue oxygenation and nutritional exchange. Measurement of microcirculation can be applied on many organs in various pathologies. In this paper we aim to review the technique of non-invasive methods for imaging of the microcirculation. Methods covered are: videomicroscopy techniques, laser Doppler perfusion imaging, and laser speckle contrast imaging. Videomicroscopy techniques, such as orthogonal polarization spectral imaging and sidestream dark-field imaging, provide a plentitude of information and offer direct visualization of the microcirculation but have the major drawback that they may give pressure artifacts. Both laser Doppler perfusion imaging and laser speckle contrast imaging allow non-contact measurements but have the disadvantage of their sensitivity to motion artifacts and that they are confined to relative measurement comparisons. Ideal would be a non-contact videomicroscopy method with fully automatic analysis software.
    Medical Devices: Evidence and Research 12/2014; 7:445-52. DOI:10.2147/MDER.S51426
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    ABSTRACT: Objective Microcirculatory visualization has already been used to investigate buccal and cutaneous microcirculatory alterations in neonates. Still, the reproducibility of these microvascular measurements has never been studied in (premature) neonates. This study aimed to determine reproducibility of the microvascular vessel density in cutaneous and buccal Sidestream Dark Field (SDF) clips in one-day old term newborns.Methods Buccal and cutaneous microcirculation was measured using SDF Imaging. Vessel density was independently assessed by two investigators. Reproducibility was assessed from the intra-class correlation coefficient (ICC) and Bland-Altman analysis.ResultsReproducibility of vessel density assessment in the buccal area was good, with ICC's for total and perfused vessel density of 0.93 (0.88-0.97) and 0.93 (95%-CI 0.85-0.97), respectively, and a near zero bias and acceptable limits of agreement in the Bland-Altman analysis. Reproducibility of assessment of the cutaneous microcirculation was poor with ICCs for total and perfused vessel density of 0.31(0-0.70) and 0.37(0-0.74), respectively, and large biases (3.09 and 2.53) in the Bland-Altman analysis.Conclusions Evaluation of buccal microvascular vessel density in SDF derived images in term newborns is reproducible in contrary to the cutaneous vessel density.This article is protected by copyright. All rights reserved.
    Microcirculation (New York, N.Y.: 1994) 08/2014; 22(1). DOI:10.1111/micc.12163 · 2.26 Impact Factor


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May 16, 2014