Elevated serum haptoglobin in pigs infected with porcine reproductive and respiratory syndrome virus.
ABSTRACT We examined the two acute phase proteins, alpha (alpha)-1 acid glycoprotein (AGP) and haptoglobin (HP), in serum of pigs following experimental porcine reproductive and respiratory syndrome (PRRS) virus infection. Increased levels of serum HP, but not AGP, were observed from 7 to 21 days post-inoculation in the infected pigs. Furthermore, serum IL-6 increased in the infected pigs, but TNF-alpha did not. The increase of serum IL-6 in pigs following PRRS virus infection may induce production of HP. Also, in the field investigation, serum HP in pigs was dramatically increased after exposure to the PRRS virus.
Article: Serum concentrations of C-reactive protein, serum amyloid A, and haptoglobin in pigs inoculated with African swine fever or classical swine fever viruses.[show abstract] [hide abstract]
ABSTRACT: To determine serum concentrations of the selected acute-phase proteins (APPs) haptoglobin, serum amyloid A (SAA), and C-reactive protein (CRP) in pigs experimentally inoculated with classical swine fever (CSF) and African swine fever (ASF) viruses. 8 crossbred (Large White x Landrace) 10-week-old pigs. Pigs were allocated to 2 groups (4 pigs/group). One group was inoculated with the CSF virus Alfort 187 strain, whereas the other groupwas inoculated with the ASF virus Spain 70 isolate. Blood samples were collected at various time points. At the end of the study, pigs were euthanized and a complete necropsy was performed, including histologic and immunohistochemical analyses. Serum concentrations of APPs increased in pigs inoculated with CSF and ASF viruses, which suggested an acute-phase response in the course of both diseases. The most noticeable increase in concentration was recorded for SAA in both groups (up to a 300-fold increase for CSF virus and an approx 40-fold increase for ASF virus), followed by CRP and then haptoglobin, which each had only 3- to 4-fold increases. Serum concentrations of APPs increased significantly in pigs inoculated with CSF and ASF viruses. However, differences were evident in serum concentrations of the proteins evaluated in this study.American Journal of Veterinary Research 08/2007; 68(7):772-7. · 1.27 Impact Factor
Article: PORCINE ACUTE PHASE PROTEIN RESPONSE TO EXPERIMENTAL INFECTION WITH BORDETELLA BRONCHISEPTICA[show abstract] [hide abstract]
ABSTRACT: The response of five positive acute phase proteins (APP) was evaluated in pigs after infection with Bordetella bronchiseptica (Bbr). Twelve piglets from a herd with high health status were used. Six of them were infected intranasally with Bbr (3.4 x 10 8 cfu). The standard bacteriological methods and PCR were used for detection of Bbr in nasal swabs, lungs, and bronchoalveolar fluids. Serum APP concentrations were measured using commercial tests. Various kinetics of response was identified within the APP tested. C-reactive protein and serum amyloid A was characterised as a very fast, transient responder, while haptoglobin as a fast and very prolonged responder. Pig major acute phase protein was characterised as a fast, protracted responder. Alfa-1-acid-glycoprotein did not respond significantly after inoculation. No correlations were found between levels of APP in serum and changes in the turbinates. On the basis of the investigation we cannot state that evaluation of APP concentrations in serum may provide useful information about severity of atrophic rhinitis. However, the concentration of most investigated APP increased after inoculation. Thus, APP measurement could be the primary screening test prior to specific disease diagnosis. "APP-high" animals may be chosen next for pathogen-specific diagnostics. Moreover, monitoring of serum APP concentrations may be useful for selecting clinically healthy pigs before integration into an uninfected herd.Bulletin- Veterinary Institute in Pulawy 01/2011; · 0.41 Impact Factor
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ABSTRACT: This study was focused on the changes observed in the serum concentration of haptoglobin (Hp), C-reactive protein (CRP), serum amyloid A (SAA) and Pig-major acute protein (Pig-MAP), during experimental porcine reproductive and respiratory syndrome virus (PRRSV) infection and in their relationship with the expression of interleukin 1β (IL-1β), interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α). Hp and Pig-MAP serum levels were increased at 10 dpi, but CRP and SAA showed a delayed and highly variable increase. All three proinflammatory cytokines were poorly expressed, and only a mild increase in IL-1β was observed at 7 dpi. The increased expression of Hp coincided with the light enhancement observed in both IL-6 and TNF-α, and might be related with an increased expression of IL-10. The low expression of TNF-α might point to a possible mechanism of viral evasion of host-immune response. This issue and the delayed expression of CRP and SAA should be taken into account in future studies about modulation of the immune response by PRRSV infection.Comparative immunology, microbiology and infectious diseases 12/2009; 33(6):e51-8. · 2.99 Impact Factor