Crystallization and preliminary diffraction analysis of the HincII restriction endonuclease-DNA complex

Department of Chemistry, Interdepartmental Program in Biochemistry and Molecular Biology, University of California at Santa Barbara, Santa Barbara CA 93106-9510, USA.
Acta Crystallographica Section D Biological Crystallography (Impact Factor: 7.23). 12/1999; 55(Pt 11):1943-5. DOI: 10.1107/S0907444999010574
Source: PubMed

ABSTRACT Crystals of the 60 kDa dimeric HincII restriction enzyme bound to a 12 base-pair dyad-symmetric duplex DNA carrying the specific 5'-GTCGAC recognition site have been obtained. Crystals grew by hanging-drop vapor diffusion from solutions containing polyethylene glycol 4000 as precipitating agent. The rod-shaped crystals belong to space group I222 (or I2(1)2(1)2(1)), with unit-cell dimensions a = 66.9, b = 176.7, c = 256.0 A. There are most likely to be two dimeric complexes in the asymmetric unit. A complete native data set has been collected from a high-energy synchrotron source to a resolution of 2.5 A at 100 K, with an R(merge) of 4.8%.

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