Article

Expression of GM3 microdomains on the surfaces of murine fibroblasts correlates with inhibition of cell proliferation.

Dipartimento di Medicina Sperimentale e Patologia, Università di Roma "La Sapienza", Viale Regina Elena 324, 00161 Rome, Italy.
Histochemie (impact factor: 2.59). 02/2000; 113(1):43-50. pp.43-50
Source: PubMed

ABSTRACT The expression and surface distribution of monosialoganglioside GM3 on the plasma membranes of NIH3T3 fibroblasts cultured at semiconfluence were analyzed by immunofluorescence as well as by immunogold electron microscopy on thin sections and surface replicas. The GM3 expression was highly variable from cell to cell and the distribution of the ganglioside on the positive cells appeared punctate. Quantitative immunogold electron microscopy showed the existence of well-defined GM3 clusters of different sizes scattered all over the cell surfaces. Double immunofluorescence analysis of 5-bromo-2'-deoxyuridine incorporation to identify proliferating cells and of GM3 expression indicated that most of the GM3-positive cells appear unable to synthesize DNA and demonstrated a growth-dependent expression of GM3.

0 0
 · 
0 Bookmarks
 · 
16 Views
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

cell surfaces
 
different sizes
 
GM3 expression
 
GM3-positive cells
 
growth-dependent expression
 
immunofluorescence
 
immunofluorescence analysis
 
immunogold electron microscopy
 
monosialoganglioside GM3
 
plasma membranes
 
positive cells
 
proliferating cells
 
Quantitative immunogold electron microscopy
 
semiconfluence
 
surface distribution
 
synthesize DNA
 
thin sections
 
well-defined GM3 clusters