Influence of Flow Patterns on Chromatographic Efficiency in Centrifugal Partition Chromatography

Laboratoire de Biochimie et Molécules Marine, IFREMER, Nantes, France.
Journal of Chromatography A (Impact Factor: 4.17). 03/2000; 869(1-2):339-52. DOI: 10.1016/S0021-9673(99)01184-X
Source: PubMed


Visualization of flow patterns in centrifugal partition chromatography (CPC) was performed with an asynchronous camera and a stroboscope triggered by the CPC rotor, allowing a channel to be selected and observed regardless of rotational speed. Three main types of flow states were noted as a function of rotational speed and flow-rate: jets stuck along channel walls, broken jets and atomization. Our observations emphasize the importance of Coriolis force on flow shape. Chromatographic efficiency was related to the dispersion of the mobile phase in the stationary phase.

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